Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Impaired Bacillus Calmette-guérin Cellular Immune Response In Hiv-exposed, Uninfected Infants
Author: Mazzola T.N.
Da Silva M.T.N.
Abramczuk B.M.
Moreno Y.M.F.
Lima S.C.B.S.
Zorzeto T.Q.
Passeto A.S.Z.
Vilela M.M.S.
Abstract: OBJECTIVE:: To evaluate cell-mediated immune response to Bacillus Calmette-Guérin (BCG) vaccination in uninfected, HIV-1-exposed infants, comparing it with unexposed children. DESIGN:: It is designed as a cross-sectional study. METHODS:: BCG-specific lymphoproliferation and T-cell subsets (CD4, CD8 and TCR γδ) by flow cytometry and interleukin-10, interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) concentration by ELISA were analyzed in HIV-exposed and unexposed infants. Whole blood lymphocyte immunophenotyping and blood counts were performed in exposed children. Nonparametric tests were used (P < 0.05). RESULTS:: Given the ontogeny of the immune system, exposed infants were separated into three groups according to age: exposed 1 (E1, aged 6.1-8.8 months), E2 (aged 9.1-17.1 months) and E3 (aged 18.1-26.3 months). Unexposed infants (UE group) and E1 were matched for age. Cell proliferation was not different among the three exposed groups, neither for BCG nor for phytohemagglutinin (PHA)-stimulated cultures. Furthermore, BCG-stimulated lymphoproliferation was reduced in the E1 group in comparison with the UE group. T-lymphocyte subpopulations also showed differences, with the youngest HIV-exposed groups (E1 and E2) showing a predominant proliferation of CD4 T cells in cultures with BCG, whereas E3 and UE groups had a robust γδ T-cell expansion. There was lower IFN-γ concentration in the samples from E1 group in comparison with all of the other groups. The unexposed infants showed higher TNF-α concentration in cultures with BCG and PHA in comparison with E1 group. CONCLUSION:: BCG-specific T-cell proliferation was reduced in HIV-exposed uninfected infants and IFN-γ concentration was lower in younger exposed infants, showing a delay in immune system maturation of HIV-exposed infants. © 2011 Wolters Kluwer Health Lippincott Williams & Wilkins.
Citation: Aids. , v. 25, n. 17, p. 2079 - 2087, 2011.
Rights: fechado
Identifier DOI: 10.1097/QAD.0b013e32834bba0a
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
2-s2.0-80055044566.pdf820.86 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.