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Type: Artigo de periódico
Title: Quantification Of Angiogenesis In Cervical Cancer: A Comparison Among Three Endothelial Cell Markers.
Author: Vieira, Sabas Carlos
Zeferino, Luiz Carlos
Da Silva, Benedito Borges
Aparecida Pinto, Glauce
Vassallo, José
Carasan, Gislaine Aparecida Fonsechi
De Moraes, Natália Guimarães
Abstract: The aim of this study was to evaluate the quantification of angiogenesis by assessing microvessel density in cervical cancer, comparing the performance of three monoclonal antibodies: anti-CD34 and BNH9, which are still little known markers in cervical neoplasia, and anti-CD31. Fifty-four consecutive patients diagnosed with stages I and II invasive cervical cancer undergoing radical hysterectomy and bilateral pelvic lymphadenectomy were evaluated. The monoclonal primary antibodies used were anti-CD34, anti-CD31 and BNH9 antibody. The areas of highest microvessel density were used for counting microvessels in 10 high-power fields. Microvessel density was the mean vessel number counted in 10 high-power fields. The mean of microvessel density was 10.3, 9.7 and 5.8 for anti-CD34, BNH9 and anti-CD31, respectively. According to the Kruskal-Wallis nonparametric test, microvessel density estimated by anti-CD34 and BNH9 was significantly higher than the microvessel density estimated by anti-CD31. The difference between microvessel density measured by anti-CD34 and BNH9 was not statistically significant. There was diagnostic agreement in 44 out of 54 cases for anti-CD34 and BNH9 monoclonal antibodies, kappa coefficient being 0.63. The kappa coefficient was 0.41 between anti-CD34 and anti-CD31 and 0.26 between anti-CD31 and BNH9. The anti-CD34 and BNH9 seem to have higher sensitivity than anti-CD31. The agreement to quantify microvessel density between anti-CD34 and BNH9 is higher than between them and anti-CD31. Additionally, considering that the BNH9 is much less unknown than anti-CD34 and that they showed very similar performance, the BNH9 clinical significance should be test in different neoplasms.
Subject: Adult
Antibodies, Monoclonal
Antigens, Cd31
Antigens, Cd34
Middle Aged
Neoplasm Staging
Neovascularization, Pathologic
Uterine Cervical Neoplasms
Citation: Gynecologic Oncology. v. 93, n. 1, p. 121-4, 2004-Apr.
Rights: fechado
Identifier DOI: 10.1016/j.ygyno.2004.01.033
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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