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Type: Artigo de periódico
Title: Gene Expression Profiles Underlying Alternative Caste Phenotypes In A Highly Eusocial Bee, Melipona Quadrifasciata.
Author: Judice, C C
Carazzole, M F
Festa, F
Sogayar, M C
Hartfelder, K
Pereira, G A G
Abstract: To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee.
Subject: Animals
Base Sequence
Expressed Sequence Tags
Gene Expression
Gene Expression Profiling
Gene Library
Juvenile Hormones
Molecular Sequence Data
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, Dna
Social Dominance
Citation: Insect Molecular Biology. v. 15, n. 1, p. 33-44, 2006-Feb.
Rights: fechado
Identifier DOI: 10.1111/j.1365-2583.2005.00605.x
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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