Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197158
Type: Artigo de periódico
Title: Gene Expression Profiles Underlying Alternative Caste Phenotypes In A Highly Eusocial Bee, Melipona Quadrifasciata.
Author: Judice, C C
Carazzole, M F
Festa, F
Sogayar, M C
Hartfelder, K
Pereira, G A G
Abstract: To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee.
Subject: Animals
Base Sequence
Bees
Expressed Sequence Tags
Female
Gene Expression
Gene Expression Profiling
Gene Library
Juvenile Hormones
Male
Molecular Sequence Data
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, Dna
Social Dominance
Citation: Insect Molecular Biology. v. 15, n. 1, p. 33-44, 2006-Feb.
Rights: fechado
Identifier DOI: 10.1111/j.1365-2583.2005.00605.x
Address: http://www.ncbi.nlm.nih.gov/pubmed/16469066
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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