Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/241964
Type: Artigo de periódico
Title: Combined Nitric Oxide-releasing Poly(vinyl Alcohol) Film/f127 Hydrogel For Accelerating Wound Healing
Author: Schanuel
Fernanda Seabra; Raggio Santos
Karen Slis; Monte-Alto-Costa
Andrea; de Oliveira
Marcelo G.
Abstract: Nitric oxide (NO) releasing biomaterials represent a potential strategy for use as active wound dressings capable of accelerating wound healing. Topical NO-releasing poly(vinyl alcohol) (PVA) films and Pluronic F127 hydrogels (F127) have already exhibited effective skin vasodilation and wound healing actions. In this study, we functionalized PVA films with SNO groups via esterification with a mixture of mercaptosucinic acid (MSA) and thiolactic acid (TLA) followed by S-nitrosation of the -SH moieties. These films were combined with an underlying layer of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), i.e., PEO-PPO-PEO (Pluronic F127) hydrogel and used for the topical treatment of skin lesions in an animal model. The mixed esterification of PVA with MSA and TLA led to chemically crosslinked PVA-SNO films with a high swelling capacity capable of spontaneously releasing NO. Real time NO-release measurements revealed that the hydrogel layer reduces the initial NO burst from the PVA-SNO films. We demonstrate that the combination of PVA-SNO films with F127 hydrogel accelerates wound contraction, decreases wound gap and cellular density and accelerates the inflammatory phase of the lesion. These results were reflected in an increase in myofibroblastic differentiation and collagen type III expression in the cicatricial tissue. Therefore, PVA-SNO films combined with F127 hydrogel may represent a new approach for active wound dressings capable of accelerating wound healing. (C) 2015 Elsevier B.V. All rights reserved.
Subject: S-nitrosothiols
Repair
Mice
Inhibition
Donors
Keratinocytes
Vasodilation
Collagen
Films
Skin
Country: AMSTERDAM
Editor: ELSEVIER SCIENCE BV
Citation: Combined Nitric Oxide-releasing Poly(vinyl Alcohol) Film/f127 Hydrogel For Accelerating Wound Healing. Elsevier Science Bv, v. 130, p. 182-191 JUN-2015.
Rights: embargo
Identifier DOI: 10.1016/j.colsurfb.2015.04.007
Address: http://www.sciencedirect.com/science/article/pii/S0927776515002131
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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