Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/242126
Type: Artigo de periódico
Title: Molecularly Imprinted Solid Phase Extraction Of Fluconazole From Pharmaceutical Formulations
Author: Manzoor
S.; Buffon
R.; Rossi
A. V.
Abstract: This work encompasses a direct and coherent strategy to synthesise a molecularly imprinted polymer (MIP) capable of extracting fluconazole from its sample. The MIP was successfully prepared from methacrylic acid (functional monomer), ethyleneglycoldimethaaylate (crosslinker) and acetonitrile (porogenic solvent) in the presence of fluconazole as the template molecule through a non-covalent approach. The non-imprinted polymer (NIP) was prepared following the same synthetic scheme, but in the absence of the template. The data obtained from scanning electronic microscopy, infrared spectroscopy, thermogravimetric and nitrogen Brunauer-Emmett-Teller plot helped to elucidate the structural as well as the morphological characteristics of the MIP and NIP. The application of MIP as a sorbent was demonstrated by packing it in solid phase extraction cartridges to extract fluconazole from commercial capsule samples through an offline analytical procedure. The quantification of fluconazole was accomplished through UPLC-MS, which resulted in LOD <= 1.63 x 10(-10) mM. Furthermore, a high percentage recovery of 91 +/- 10% (n=9) was obtained. The ability of the MIP for selective recognition of fluconazole was evaluated by comparison with the structural analogues, miconazole, tioconazole and secnidazole, resulting in percentage recoveries of 51, 35 and 32%, respectively. (C) 2014 Elsevier B.V. All rights reserved.
Subject: Liquid-chromatography
Selective Extraction
Human Plasma
Polymer Microspheres
Samples
Performance
Separation
Sorbents
Caffeine
Acid
Country: AMSTERDAM
Editor: ELSEVIER SCIENCE BV
Citation: Molecularly Imprinted Solid Phase Extraction Of Fluconazole From Pharmaceutical Formulations. Elsevier Science Bv, v. 134, p. 1-7 MAR-2015.
Rights: embargo
Identifier DOI: 10.1016/j.talanta.2014.10.057
Address: http://www.sciencedirect.com/science/article/pii/S0039914014008856
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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