Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328340
Type: Artigo
Title: Anti-inflammatory Activity And Polyphenolic Profile Of The Hydroalcoholic Seed Extract Of Eugenia Leitonii, An Unexplored Brazilian Native Fruit
Anti-inflammatory activity and polyphenolic profile of the hydroalcoholic seed extract of Eugenia leitonii, an unexplored brazilian native fruit
Author: Lazarini, Josy Goldoni
Franchin, Marcelo
Infante, Juliana
Rizzato Paschoal, Jonas Augusto
Freires, Irlan Almeida
de Alencar, Severino Matias
Rosalen, Pedro Luiz
Abstract: This study determined the anti-inflammatory activity, mechanisms of action, and polyphenolic profile of Eugenia leitonii seed extract. The total phenolic content of the hydroethanolic extract from E. leitonii seeds (ESEL) was measured, and its phenolic compounds were identified by LC-MS/MS. The ESEL was submitted to in vitro (RAW264.7) and in vivo assays on neutrophil migration; expression of P-selectin and ICAM-1; release of TNF-alpha, IL-1 beta, and CXCL2/ MIP-2; NF-kappa B activation; and carrageenan-induced paw oedema. ESEL yielded total phenolic content of 158.74 +/- 3.5 mg GAE/g, with the presence of flavonols, anthocyanins and ellagitannins. ESEL significantly reduced neutrophil migration; decreased the expression of P-selectin and ICAM-1; decreased the release of TNF-alpha and CXCL2/MIP-2, as well as NF-kappa B activation and paw oedema in mice. Thus, this extract showed to be a promising source of bioactive compounds with anti-inflammatory potential by inhibiting neutrophil migration through a decrease in the release of TNF-alpha and CXCL2/MIP-2 (NF-kappa B pathway) in macrophages. (C) 2016 Elsevier Ltd. All rights reserved.
This study determined the anti-inflammatory activity, mechanisms of action, and polyphenolic profile of Eugenia leitonii seed extract. The total phenolic content of the hydroethanolic extract from E. leitonii seeds (ESEL) was measured, and its phenolic compounds were identified by LC-MS/MS. The ESEL was submitted to in vitro (RAW264.7) and in vivo assays on neutrophil migration; expression of P-selectin and ICAM-1; release of TNF-alpha, IL-1 beta, and CXCL2/ MIP-2; NF-kappa B activation; and carrageenan-induced paw oedema. ESEL yielded total phenolic content of 158.74 +/- 3.5 mg GAE/g, with the presence of flavonols, anthocyanins and ellagitannins. ESEL significantly reduced neutrophil migration; decreased the expression of P-selectin and ICAM-1; decreased the release of TNF-alpha and CXCL2/MIP-2, as well as NF-kappa B activation and paw oedema in mice. Thus, this extract showed to be a promising source of bioactive compounds with anti-inflammatory potential by inhibiting neutrophil migration through a decrease in the release of TNF-alpha and CXCL2/MIP-2 (NF-kappa B pathway) in macrophages
Subject: Anti-inflammatory
Eugenia Leitonii
Native Fruits
Phenolic Compounds
Cytokines
Citocinas
Compostos fenólicos
Anti-inflamatórios
Country: Países Baixos
Editor: Elsevier
Citation: Journal Of Functional Foods. Elsevier Science Bv, v. 26, p. 249 - 257, 2016.
Rights: fechado
Identifier DOI: 10.1016/j.jff.2016.08.001
Address: https://www.sciencedirect.com/science/article/pii/S1756464616302092
Date Issue: 2016
Appears in Collections:FOP - Artigos e Outros Documentos

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