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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleA transcript finishing initiative for closing gaps in the human transcriptomept_BR
dc.contributor.authorSogayar, MCpt_BR
dc.contributor.authorCamargo, AApt_BR
dc.contributor.authorBettoni, Fpt_BR
dc.contributor.authorCarraro, DMpt_BR
dc.contributor.authorPires, LCpt_BR
dc.contributor.authorParmigiani, MBpt_BR
dc.contributor.authorFerreira, ENpt_BR
dc.contributor.authorMoreira, ESpt_BR
dc.contributor.authorLatorre, MRDOpt_BR
dc.contributor.authorSimpson, AJGpt_BR
dc.contributor.authorCruz, LOpt_BR
dc.contributor.authorDegaki, TLpt_BR
dc.contributor.authorFesta, Fpt_BR
dc.contributor.authorMassirer, KBpt_BR
dc.contributor.authorCamargo, Fpt_BR
dc.contributor.authorCamargo, LPpt_BR
dc.contributor.authorCunha, MAVpt_BR
dc.contributor.authorDe Souza, SJpt_BR
dc.contributor.authorFaria, Mpt_BR
dc.contributor.authorGiuliatti, Spt_BR
dc.contributor.authorKopppt_BR
dc.contributor.authorOliviera, PSLpt_BR
dc.contributor.authorPaiva, PBpt_BR
dc.contributor.authorPereira, AApt_BR
dc.contributor.authorPinheiro, DGpt_BR
dc.contributor.authorPuga, RDpt_BR
dc.contributor.authorSouza, JESpt_BR
dc.contributor.authorAlbuquerque, DMpt_BR
dc.contributor.authorAndrade, LECpt_BR
dc.contributor.authorBaia, GSpt_BR
dc.contributor.authorBriones, MRSpt_BR
dc.contributor.authorCavaleiro-Luna, AMSpt_BR
dc.contributor.authorCerutti, JMpt_BR
dc.contributor.authorCosta, FFpt_BR
dc.contributor.authorConstanzi-Strauss, Ept_BR
dc.contributor.authorEspreafico, EMpt_BR
dc.contributor.authorFerrasi, ACpt_BR
dc.contributor.authorFerro, ESpt_BR
dc.contributor.authorFortes, MAHZpt_BR
dc.contributor.authorFurchi, JRFpt_BR
dc.contributor.authorGianella-Neto, Dpt_BR
dc.contributor.authorGoldman, GHpt_BR
dc.contributor.authorGoldman, MHSpt_BR
dc.contributor.authorGruber, Apt_BR
dc.contributor.authorGuimaraes, GSpt_BR
dc.contributor.authorHackel, Cpt_BR
dc.contributor.authorHenrique-Silva, Fpt_BR
dc.contributor.authorKimura, ETpt_BR
dc.contributor.authorLeoni, SGpt_BR
dc.contributor.authorMacedo, Cpt_BR
dc.contributor.authorMalnic, Bpt_BR
dc.contributor.authorManzini, CVpt_BR
dc.contributor.authorMarie, SKNpt_BR
dc.contributor.authorMartinez-Rossi, NMpt_BR
dc.contributor.authorMenossi, Mpt_BR
dc.contributor.authorMiracca, ECpt_BR
dc.contributor.authorNagai, MApt_BR
dc.contributor.authorNobrega, FGpt_BR
dc.contributor.authorNobrega, MPpt_BR
dc.contributor.authorOba-Shinjo, SMpt_BR
dc.contributor.authorOliviera, MKpt_BR
dc.contributor.authorOrabona, GMpt_BR
dc.contributor.authorOtsuke, AYpt_BR
dc.contributor.authorPaco-Larson, MLpt_BR
dc.contributor.authorPaixao, BMCpt_BR
dc.contributor.authorPandolfi, JRCpt_BR
dc.contributor.authorPardini, MIMCpt_BR
dc.contributor.authorPassos-Bueno, MRpt_BR
dc.contributor.authorPassos, GASpt_BR
dc.contributor.authorPesquero, JBpt_BR
dc.contributor.authorPessoa, JGpt_BR
dc.contributor.authorRahal, Ppt_BR
dc.contributor.authorRainho, CApt_BR
dc.contributor.authorReis, CPpt_BR
dc.contributor.authorRicca, TIpt_BR
dc.contributor.authorRodriguez, Vpt_BR
dc.contributor.authorRogatto, SRpt_BR
dc.contributor.authorRomano, CMpt_BR
dc.contributor.authorRomeiro, JGpt_BR
dc.contributor.authorRossi, Apt_BR
dc.contributor.authorSa, RGpt_BR
dc.contributor.authorSales, MMpt_BR
dc.contributor.authorSantAnna, SCpt_BR
dc.contributor.authorSantarosa, PLpt_BR
dc.contributor.authorSegato, Fpt_BR
dc.contributor.authorSilva, WApt_BR
dc.contributor.authorSilva, IDCGpt_BR
dc.contributor.authorSilva, NPpt_BR
dc.contributor.authorSoares-Costa, Apt_BR
dc.contributor.authorSonati, MFpt_BR
dc.contributor.authorStrauss, BEpt_BR
dc.contributor.authorTajara, EHpt_BR
dc.contributor.authorValentini, SRpt_BR
dc.contributor.authorVillanova, FEpt_BR
dc.contributor.authorWard, LSpt_BR
dc.contributor.authorZanette, DLpt_BR
unicamp.author.emailmcsoga@lq.usp.brpt_BR
unicamp.author.emailanamaria@compblo.ludwig.org.brpt_BR
unicamp.authorUniv Sao Paulo, Inst Quim, BR-05513970 Sao Paulo, Brazil Ludwig Inst Canc Res, BR-01509010 Sao Paulo, Brazil Univ Sao Paulo, Dept Epidemiol, Sch Publ Hlth, BR-01246904 Sao Paulo, SP, Brazil Univ Ribeirao Preto, Dept Quim Informat Bioinformat, BR-14096380 Ribeirao Preto, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Centro Terapia Celular, Hemocentro, BR-14051140 Ribeirao Preto, SP, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, BR-14051140 Ribeirao Preto, SP, Brazil Inst Ludwig, Lab Biol Computacional, BR-01509010 Sao Paulo, SP, Brazil Univ Sao Paulo, Lab Genet Cardiol Mol, Inst Coracao, BR-05403000 Sao Paulo, SP, Brazil Univ Fed Sao Paulo, Bioinform Lab, Hlth Informatics Dept, BR-04039032 Sao Paulo, Brazil Univ Estad Campinas, Dept Clin Med, Fac Ciencias Med, BR-13083970 Campinas, SP, Brazil Univ Fed Sao Paulo, Rheumatol Div, BR-04113001 Sao Paulo, Brazil Univ Sao Paulo, Dept Histol Embriol, Inst Ciencias Biomed, BR-05508900 Sao Paulo, Brazil Univ Fed Sao Paulo, Dept Microbiol Immunol Parasitol, BR-04023062 Sao Paulo, Brazil Univ Sao Paulo, Lab Cellular Mol Endocrinol, Sch Med, BR-01246903 Sao Paulo, Brazil Univ Fed Sao Paulo, Dept Med, Disciplina Endocrinol, Lab Endocrinol Mol, BR-04039002 Sao Paulo, Brazil Univ Sao Paulo, Inst Ciencias Biomed, Lab Transferencia Genica, BR-05508900 Sao Paulo, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular Mol Bioagentes Patogenicas, BR-14049900 Ribeirao Preto, SP, Brazil Univ Estadual Paulista, Lab Biol Mol, Hemocentro, Fac Med, BR-18618970 Botucatu, SP, Brazil Univ Fed Sao Carlos, Dept Genet Evol, BR-13565905 Sao Carlos, SP, Brazil Univ Sao Paulo, Fac Ciencias Farmaceuticas Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil Univ Sao Paulo, Fac Filosofia Ciencias Letras Ribeirao Preto, Ribeirao Preto, SP, Brazil Univ Sao Paulo, Dept Patol, Fac Med Vet Zootecnia, BR-05508000 Sao Paulo, Brazil Univ Estadual Campinas, Dept Genet Med, Fac Ciencias Med, BR-13081970 Campinas, SP, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040900 Ribeirao Preto, SP, Brazil Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05599970 Sao Paulo, Brazil Univ Sao Paulo, Dept Neurol, Fac Med, BR-01246903 Sao Paulo, Brazil Univ Estadual Campinas, Lab Genoma Funcional, Centro Biol Mol Engenharia Genet, BR-13083970 Campinas, SP, Brazil Univ Estadual Campinas, Dept Genet Evol, Inst Biol, Campinas, SP, Brazil Univ Sao Paulo, Dept Radiol, Disciplina Oncol, Fac Med, BR-01246903 Sao Paulo, Brazil Univ Vale Paraiba, Inst Pesquisa Desenvolvimento, BR-12244000 Sao Jose Dos Campos, SP, Brazil Univ Sao Paulo, Inst Biociencias, Centro Estudios Genoma Humano, Dept Biol, BR-055508900 Sao Paulo, Brazil Univ Fed Sao Paulo, Lab Mol Gynecol, Dept Gynecol, BR-04039001 Sao Paulo, Brazil Sao Paulo State Univ, Sch Pharmacol, Dept Biol Sci, BR-14801902 Araraquara, SP, Brazil Univ Sao Paulo, Discipl Genet, Fac Odontol, BR-14040900 Ribeirao Preto, SP, Brazil Univ Fed Sao Paulo, Dept Biofisica, BR-04023062 Sao Paulo, Brazil Univ Estadual Paulista, Dept Biol, Inst Biociencias Letras Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil Univ Estadual Paulista, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil Univ Sao Paulo, Dept Bioquim Imunol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil Univ Estadual Campinas, Lab Genet Mol Cancer, Dept Clin Med, Fac Ciencias Med, BR-13083970 Campinas, SP, Brazil Univ Estadual Campinas, Dept Patol Clin, Fac Ciencias Med, BR-13083970 Campinas, SP, Brazil Univ Sao Paulo, Setor Vetores Virals, Lab Cardiol Mol, Inst Coracao, BR-15403000 Sao Paulo, Brazilpt_BR
dc.subject.wosExpressed Sequence Tagspt_BR
dc.subject.wosSingle-nucleotide Polymorphismspt_BR
dc.subject.wosHuman Messenger-rnaspt_BR
dc.subject.wosGenome Anatomy Projectpt_BR
dc.subject.wosHuman Genespt_BR
dc.subject.wosDna-sequencept_BR
dc.subject.wosAlternate Polyadenylationpt_BR
dc.subject.wosComputational Analysispt_BR
dc.subject.wosWide Detectionpt_BR
dc.subject.wosHuman Brainpt_BR
dc.description.abstractWe report the results of a transcript finishing initiative, undertaken for the purpose of identifying and characterizing novel human transcripts, in which RT-PCR was used to bridge gaps between paired EST Clusters, mapped against the genomic sequence. Each pair of EST Clusters selected for experimental validation was designated a transcript finishing unit (TFU). A total of 489 TFUs were selected for validation, and an overall efficiency of 43.1% was achieved. We generated a total of 59,975 bp of transcribed sequences organized into 432 exons, contributing to the definition of the structure of 211 human transcripts. The structure of several transcripts reported here was confirmed during the course of this project, through the generation of their corresponding full-length cDNA sequences. Nevertheless, for 21% of the validated TFUs, a full-length cDNA sequence is not yet available in public databases, and the structure of 69.2% of these TFUs was not correctly predicted by computer programs. The TF strategy provides a significant contribution to the definition of the complete catalog of human genes and transcripts, because it appears to be particularly useful for identification of low abundance transcripts expressed in a restricted Set Of tissues as well as for the delineation of gene boundaries and alternatively spliced isoforms.pt
dc.description.noteO TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE FEVEREIRO DE 2015.pt
dc.relation.ispartofGenome Researchpt_BR
dc.relation.ispartofabbreviationGenome Res.pt_BR
dc.publisher.cityCold Spring Harborpt_BR
dc.publisher.countryEUApt_BR
dc.publisherCold Spring Harbor Lab Press, Publications Deptpt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculationJULpt_BR
dc.identifier.citationGenome Research. Cold Spring Harbor Lab Press, Publications Dept, v. 14, n. 7, n. 1413, n. 1423, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume14pt_BR
dc.description.issuenumber7pt_BR
dc.description.firstpage1413pt_BR
dc.description.lastpage1423pt_BR
dc.rightsembargopt_BR
dc.sourceWeb of Sciencept_BR
unicamp.cruespUNESP USPpt_BR
dc.identifier.issn1088-9051pt_BR
dc.identifier.wosidWOS:000222434200023pt_BR
dc.identifier.doi10.1101/gr.2111304pt_BR
dc.date.available2014-11-16T22:22:14Z
dc.date.available2015-11-26T16:25:36Z-
dc.date.accessioned2014-11-16T22:22:14Z
dc.date.accessioned2015-11-26T16:25:36Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:25:36Z (GMT). No. of bitstreams: 2 WOS000222434200023.pdf: 385255 bytes, checksum: cbad5c19707956503f78c57365b05e70 (MD5) WOS000222434200023.pdf.txt: 69113 bytes, checksum: c76869b6243a4d46f736654158988d9d (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/53935pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/53935
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/53935-
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