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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleAcidocalcisomes of Phytomonas francai possess distinct morphological characteristics and contain ironpt_BR
dc.contributor.authorMiranda, Kpt_BR
dc.contributor.authorRodrigues, COpt_BR
dc.contributor.authorHentchel, Jpt_BR
dc.contributor.authorVercesi, Apt_BR
dc.contributor.authorPlattner, Hpt_BR
dc.contributor.authorde Souza, Wpt_BR
dc.contributor.authorDocampo, Rpt_BR
unicamp.author.emailrodoc@staff.uiuc.edupt_BR
unicamp.authorFed Univ Rio De Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestructura Celular Hertha Meyer, BR-21949900 Rio De Janeiro, Brazil Univ Illinois, Mol Parasitol Lab, Dept Pathobiol, Urbana, IL 61802 USA Univ Illinois, Ctr Zoonoses Res, Urbana, IL 61802 USA Univ Konstanz, Dept Biol, D-78457 Constance, Germany Univ Estadual Campinas, Lab Bioenerget Nucl Med & Cirurgia Expt, Campinas, SP, Brazilpt_BR
dc.subjectPhytomonaspt_BR
dc.subjectacidocalcisomespt_BR
dc.subjectelemental mappingpt_BR
dc.subjectpyrophosphatasept_BR
dc.subjectcalciumpt_BR
dc.subjecttransmission electron microscopypt_BR
dc.subject.wosVacuolar H+-pyrophosphatasept_BR
dc.subject.wosCruzi Mitochondria Insitupt_BR
dc.subject.wosTrypanosoma-cruzipt_BR
dc.subject.wosIntracellular Calciumpt_BR
dc.subject.wosElemental Compositionpt_BR
dc.subject.wosLeishmania-donovanipt_BR
dc.subject.wosNa+/h+ Exchangerpt_BR
dc.subject.wosPoor Developmentpt_BR
dc.subject.wosCa-2+ Transportpt_BR
dc.subject.wosFine-structurept_BR
dc.description.abstractAcidocalcisomes are acidic calcium storage compartments described initially in trypanosomatid and apicomplexan parasites, and recently found in other unicellular eukaryotes. The aim of this study was to identify the presence of acidocalcisomes in the plant trypanosomatid Phytomonas francai. Electron-dense organelles of P francai were shown to contain large amounts of oxygen, sodium, magnesium, phosphorus, potassium, calcium, iron, and zinc as determined by X-ray microanalysis, either in situ or when purified using iodixanol gradient centrifugation or by elemental mapping. The presence of iron is not common in other acidocalcisomes. In situ, but not when purified, these organelles showed an elongated shape differing from previously described acidocalcisomes. However, these organelles also possessed a vacuolar H+-pyrophosphatase (V-H+-PPase) as determined by biochemical methods and by immunofluorescence microscopy using antibodies against the enzyme. Together, these results suggest that the electron-dense organelles of P. francai are homologous to the acidocalcisomes described in other trypanosomatids, although with distinct morphology and elemental content.pt
dc.description.noteo TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.pt
dc.relation.ispartofMicroscopy And Microanalysispt_BR
dc.relation.ispartofabbreviationMicrosc. microanal.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisher.countryEUApt_BR
dc.publisherCambridge Univ Presspt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculationOCTpt_BR
dc.identifier.citationMicroscopy And Microanalysis. Cambridge Univ Press, v. 10, n. 5, n. 647, n. 655, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume10pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage647pt_BR
dc.description.lastpage655pt_BR
dc.rightsembargopt_BR
dc.rights.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676pt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1431-9276pt_BR
dc.identifier.wosidWOS:000224197000012pt_BR
dc.identifier.doi10.1017/S1431927604040887pt_BR
dc.date.available2014-11-16T11:35:30Z
dc.date.available2015-11-26T16:22:36Z-
dc.date.accessioned2014-11-16T11:35:30Z
dc.date.accessioned2015-11-26T16:22:36Z-
dc.description.provenanceMade available in DSpace on 2014-11-16T11:35:30Z (GMT). No. of bitstreams: 1 WOS000224197000012.pdf: 436669 bytes, checksum: e183c40858794068ab2e420f25248e37 (MD5) Previous issue date: 2004en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:22:36Z (GMT). No. of bitstreams: 2 WOS000224197000012.pdf: 436669 bytes, checksum: e183c40858794068ab2e420f25248e37 (MD5) WOS000224197000012.pdf.txt: 36896 bytes, checksum: ed468f1e4407289568462d7c15490b54 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54119pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/54119
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54119-
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