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Type: Artigo de periódico
Title: Analysis of V gamma/J beta trans-rearrangements in paediatric patients undergoing chemotherapy
Author: Lopes, LF
Neto, ED
Lorand-Metze, I
Latorre, MDDO
Simpson, AJG
Abstract: The frequency of the hybrid V gamma /J beta trans-rearrangement in peripheral blood lymphocytes (PBLs) was analysed in a transversal study of paediatric patients (n = 210) with acute lymphoblastic leukaemia (ALL) and solid tumours (ST). Different amounts of DNA were used as the template for a nested polymerase chain reaction to evaluate the frequency of hybrid V gamma /J beta genes, using silver-stained gels. The frequency of the rearrangement was evaluated in groups before, during and after therapy. A greatly increased frequency of V gamma /J beta trans-rearrangement was found in PBLs of both groups of patients during exposure to chemotherapeutic agents compared with patients before chemotherapy. In patients who had finished treatment, the frequency of the rearrangement fell promptly to the baseline levels in ST but showed a slow decrease in ALL in those in whom increased levels could be found until 4 years after the end of treatment. We hypothesize that the chemotherapeutic agents are able to induce the V gamma /J beta trans-rearrangement, but this is transient in most cases. The exact relationship between the persistence of the rearrangement and the occurrence of secondary leukaemia remains to be determined.
Subject: V gamma/J beta gene
paediatric cancer
childhood secondary leukaemia
V gamma/J beta gene chemotherapy
V gamma/J beta trans-rearrangement
Country: Inglaterra
Editor: Blackwell Science Ltd
Citation: British Journal Of Haematology. Blackwell Science Ltd, v. 113, n. 4, n. 1001, n. 1008, 2001.
Rights: fechado
Identifier DOI: 10.1046/j.1365-2141.2001.02826.x
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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