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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlec-Jun is regulated by combination of enhanced expression and phosphorylation in acute-overloaded rat heartpt_BR
dc.contributor.authorNadruz, Wpt_BR
dc.contributor.authorKobarg, CBpt_BR
dc.contributor.authorKobarg, Jpt_BR
dc.contributor.authorFranchini, KGpt_BR
unicamp.author.emailfranchin@obelix.unicamp.brpt_BR
unicamp.authorState Univ Campinas, Sch Med, Dept Internal Med, BR-13081970 Campinas, SP, Brazil Natl Synchrotron Light Lab, Ctr Struct Mol Biol, BR-13084971 Campinas, SP, Brazilpt_BR
dc.subjectpressure overloadpt_BR
dc.subjectproteinpt_BR
dc.subjectmyocardiumpt_BR
dc.subjectactivator protein-1pt_BR
dc.subject.wosCardiac Gene-expressionpt_BR
dc.subject.wosPressure-overloadpt_BR
dc.subject.wosMechanical-stresspt_BR
dc.subject.wosProtooncogene Inductionpt_BR
dc.subject.wosNuclear Translocationpt_BR
dc.subject.wosNh2-terminal Kinasespt_BR
dc.subject.wosPositive Regulationpt_BR
dc.subject.wosIn-vivopt_BR
dc.subject.wosHypertrophypt_BR
dc.subject.wosProteinpt_BR
dc.description.abstractThe transient increase in the expression of transcription factors encoded by immediate- early genes has been considered to play a critical role in the coordination of early gene expression during the hypertrophic growth of cardiac myocytes. Here, we investigated the regulation of c- Jun and its upstream activators JNKs in the myocardium of rats subjected to acute pressure overload induced by transverse aortic constriction. Western blotting and immunohistochemistry analysis demonstrated that both JNK1 and JNK2 were transiently activated by pressure overload, but only JNK1 was activated at the nuclei of cardiac myocytes. JNK1 activation was paralleled by phosphorylation of c- Jun at serine- 63 in the myocardial nuclear fraction and by an increase in c- Jun expression in cardiac myocytes. A consistent increase in DNA binding of activator protein-1 ( AP- 1) complex was observed after 10 and 30 min of pressure overload and Supershift assays confirmed that c- Jun was a major component of activated AP- 1 complex. Moreover, experiments performed with the specific JNK inhibitor SP- 600125 abolished c- Jun phosphorylation and markedly attenuated its expression as well as the expression of the fetal gene beta- myosin heavy chain. Overall, these findings demonstrate a molecular basis for load- induced activation of c- Jun in cardiac myocytes and its connection with the regulation of fetal gene, characteristic of the acute response to pressure overload.pt
dc.description.noteo TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.pt
dc.relation.ispartofAmerican Journal Of Physiology-heart And Circulatory Physiologypt_BR
dc.relation.ispartofabbreviationAm. J. Physiol.-Heart Circul. Physiol.pt_BR
dc.publisher.cityBethesdapt_BR
dc.publisher.countryEUApt_BR
dc.publisherAmer Physiological Socpt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculationFEB 1pt_BR
dc.identifier.citationAmerican Journal Of Physiology-heart And Circulatory Physiology. Amer Physiological Soc, v. 286, n. 2, n. H760, n. H767, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume286pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpageH760pt_BR
dc.description.lastpageH767pt_BR
dc.rightsembargopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0363-6135pt_BR
dc.identifier.wosidWOS:000187911900034pt_BR
dc.identifier.doi10.1152/ajpheart.00430.2003pt_BR
dc.date.available2014-11-15T16:30:16Z
dc.date.available2015-11-26T16:12:00Z-
dc.date.accessioned2014-11-15T16:30:16Z
dc.date.accessioned2015-11-26T16:12:00Z-
dc.description.provenanceMade available in DSpace on 2014-11-15T16:30:16Z (GMT). No. of bitstreams: 1 WOS000187911900034.pdf: 468210 bytes, checksum: d11b426211da9dfce48d0b7b6c694536 (MD5) Previous issue date: 2004en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:12:00Z (GMT). No. of bitstreams: 2 WOS000187911900034.pdf: 468210 bytes, checksum: d11b426211da9dfce48d0b7b6c694536 (MD5) WOS000187911900034.pdf.txt: 42615 bytes, checksum: 0d3165d593c21daa6d59391cf9969fa3 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55948pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/55948
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55948-
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