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Type: Artigo de periódico
Title: Conservation and versatility of a new set of primers for long-PCR amplification of complete insect mitochondrial genomes based on Haematobia irritans mtDNA sequences
Author: Barau, JG
De Azeredo-Espin, AML
Lessinger, AC
Abstract: The amplification of complete mitochondrial genomes by long PCR (polymerase chain reaction) has been a major contribution to the large-scale sequencing of arthropodan mitochondrial genomes. In this work, we designed six conserved long-PCR primers to successfully recover the entire mitochondrial genome of the horn fly Haematobia irritans (Diptera: Muscidae) in two overlapping fragments. The conservation and versatility of these primers were tested for 17 other species from four major insect orders: Diptera (14), Coleoptera (1), Lepidoptera (1) and Hymenoptera (1). The amplification of complete mitochondrial genomes in orders other than Diptera suggested an even broader application of these primers, especially within the Hexapoda.
Subject: Haematobia irritans
long PCR
mitochondrial genome
Country: Inglaterra
Editor: Blackwell Publishing
Citation: Molecular Ecology Notes. Blackwell Publishing, v. 5, n. 4, n. 885, n. 887, 2005.
Rights: fechado
Identifier DOI: 10.1111/j.1471-8286.2005.01103.x
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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