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Type: Artigo de periódico
Title: Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase
Author: Kiyota, E
de Sousa, SM
dos Santos, ML
Lima, AD
Menossi, M
Yunes, JA
Aparicio, R
Abstract: Maize aldose reductase (AR) is a member of the aldo-keto reductase superfamily. In contrast to human AR, maize AR seems to prefer the conversion of sorbitol into glucose. The apoenzyme was crystallized in space group P2(1)2(1)2(1), with unit-cell parameters a = 47.2, b = 54.5, c = 100.6 angstrom and one molecule in the asymmetric unit. Synchrotron X-ray diffraction data were collected and a final resolution limit of 2.0 angstrom was obtained after data reduction. Phasing was carried out by an automated molecular-replacement procedure and structural refinement is currently in progress. The refined structure is expected to shed light on the functional/enzymatic mechanism and the unusual activities of maize AR.
Country: Inglaterra
Editor: Blackwell Publishing
Citation: Acta Crystallographica Section F-structural Biology And Crystallization Communications. Blackwell Publishing, v. 63, n. 990, n. 992, 2007.
Rights: aberto
Identifier DOI: 10.1107/S1744309107052670
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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