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Type: Artigo de periódico
Title: RAPD genomic fingerprinting differentiates Thiobacillus ferrooxidans strains
Author: Novo, MTM
DeSouza, AP
Garcia, O
Ottoboni, LMM
Abstract: The PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was optimized and used for assessing genomic variability among eight Thiobacillus ferrooxidans strains. RAPD fingerprints presented variation for the thirty primers used, giving a total of 269 polymorphic bands. Similarity coefficients between the strains were calculated, and UPGMA cluster analysis was used to generate a dendrogram showing relationships among them. Most primers divided T. ferrooxidans strains in two distinct groups - Group 1: S, SSP, V3, AMF and Group 2: CMV, FG-460, I-35, LR. We observed that the T. ferrooxidans strains used in this work have a high degree of genomic diversity and that RAPD is a powerful method to differentiate them.
Subject: Thiobacillus ferrooxidans, RAPD
genomic fingerprinting
Country: Alemanha
Editor: Gustav Fischer Verlag
Citation: Systematic And Applied Microbiology. Gustav Fischer Verlag, v. 19, n. 1, n. 91, n. 95, 1996.
Rights: fechado
Date Issue: 1996
Appears in Collections:Unicamp - Artigos e Outros Documentos

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