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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePIP4KIIA and beta-globin: transcripts differentially expressed in reticulocytes and associated with high levels of Hb H in two siblings with Hb H diseasept_BR
dc.contributor.authorWenning, MRSCpt_BR
dc.contributor.authorMello, MPpt_BR
dc.contributor.authorAndrade, TGpt_BR
dc.contributor.authorLanaro, Cpt_BR
dc.contributor.authorAlbuquerque, DMpt_BR
dc.contributor.authorSaad, STOpt_BR
dc.contributor.authorCosta, FFpt_BR
dc.contributor.authorSonati, MFpt_BR
unicamp.authorWenning, Marcia R. S. C. Sonati, Maria F. Univ Estadual Campinas, Dept Clin Pathol, Sch Med Sci, UNICAMP, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorMello, Maricilda P. Univ Estadual Campinas, Mol Biol & Genet Ctr, CBMEG, UNICAMP, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorAndrade, Tiago G. Lanaro, Carolina Albuquerque, Dulcineia M. Saad, Sara T. O. Costa, Fernando F. Univ Estadual Campinas, Hematol & Hemotherapy Ctr, UNICAMP, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectPIP kinasept_BR
dc.subjectglobin genespt_BR
dc.subjectHb H diseasept_BR
dc.subjectdifferential gene expressionpt_BR
dc.subject.wosPhosphatidylinositol Phosphate Kinasespt_BR
dc.description.abstractWe are reporting here the results of differential gene expression experiments comparing two siblings, a 21-yr-old male and a 19-yr-old female, with the same alpha-thalassemia genotype (-alpha 3.7/--SEA) and quite different levels of Hb H in the peripheral blood (18.7 and 5%, respectively). By using mRNA differential-display reverse-transcription-PCR and suppression subtractive hybridization, two main transcripts were selected in both procedures and validated by qRT-PCR, one corresponding to the phosphatidylinositol phosphate 4-kinase type II-alpha (PIP4KIIA) gene and the other to the beta-globin gene, both over expressed in the patient with the higher percentage of Hb H. Type II PIP kinases produce phosphatidylinositol 4,5 biphosphate, a critical and pleiotropic regulatory molecule involved in diverse cellular activities, including gene expression. Our results suggest that PIP4KIIA may be one of the factors related to the regulation of the beta-globin gene expression and the different levels of Hb H in alpha-thalassemic
dc.relation.ispartofEuropean Journal Of Haematologypt_BR
dc.relation.ispartofabbreviationEur. J. Haematol.pt_BR
dc.identifier.citationEuropean Journal Of Haematology. Wiley-blackwell, v. 83, n. 5, n. 490, n. 493, 2009.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsordocumentnumberFAPESP [02/13801-7]pt
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