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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePIP4KIIA and beta-globin: transcripts differentially expressed in reticulocytes and associated with high levels of Hb H in two siblings with Hb H diseasept_BR
dc.contributor.authorWenning, MRSCpt_BR
dc.contributor.authorMello, MPpt_BR
dc.contributor.authorAndrade, TGpt_BR
dc.contributor.authorLanaro, Cpt_BR
dc.contributor.authorAlbuquerque, DMpt_BR
dc.contributor.authorSaad, STOpt_BR
dc.contributor.authorCosta, FFpt_BR
dc.contributor.authorSonati, MFpt_BR
unicamp.author.emailsonati@fcm.unicamp.brpt_BR
unicamp.authorWenning, Marcia R. S. C. Sonati, Maria F. Univ Estadual Campinas, Dept Clin Pathol, Sch Med Sci, UNICAMP, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorMello, Maricilda P. Univ Estadual Campinas, Mol Biol & Genet Ctr, CBMEG, UNICAMP, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorAndrade, Tiago G. Lanaro, Carolina Albuquerque, Dulcineia M. Saad, Sara T. O. Costa, Fernando F. Univ Estadual Campinas, Hematol & Hemotherapy Ctr, UNICAMP, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectPIP kinasept_BR
dc.subjectglobin genespt_BR
dc.subjectalpha-thalassemiapt_BR
dc.subjectHb H diseasept_BR
dc.subjectdifferential gene expressionpt_BR
dc.subject.wosPhosphatidylinositol Phosphate Kinasespt_BR
dc.subject.wosFamilypt_BR
dc.description.abstractWe are reporting here the results of differential gene expression experiments comparing two siblings, a 21-yr-old male and a 19-yr-old female, with the same alpha-thalassemia genotype (-alpha 3.7/--SEA) and quite different levels of Hb H in the peripheral blood (18.7 and 5%, respectively). By using mRNA differential-display reverse-transcription-PCR and suppression subtractive hybridization, two main transcripts were selected in both procedures and validated by qRT-PCR, one corresponding to the phosphatidylinositol phosphate 4-kinase type II-alpha (PIP4KIIA) gene and the other to the beta-globin gene, both over expressed in the patient with the higher percentage of Hb H. Type II PIP kinases produce phosphatidylinositol 4,5 biphosphate, a critical and pleiotropic regulatory molecule involved in diverse cellular activities, including gene expression. Our results suggest that PIP4KIIA may be one of the factors related to the regulation of the beta-globin gene expression and the different levels of Hb H in alpha-thalassemic patients.pt
dc.relation.ispartofEuropean Journal Of Haematologypt_BR
dc.relation.ispartofabbreviationEur. J. Haematol.pt_BR
dc.publisher.cityMaldenpt_BR
dc.publisher.countryEUApt_BR
dc.publisherWiley-blackwellpt_BR
dc.date.issued2009pt_BR
dc.date.monthofcirculationNOVpt_BR
dc.identifier.citationEuropean Journal Of Haematology. Wiley-blackwell, v. 83, n. 5, n. 490, n. 493, 2009.pt_BR
dc.language.isoenpt_BR
dc.description.volume83pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage490pt_BR
dc.description.lastpage493pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlpt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0902-4441pt_BR
dc.identifier.wosidWOS:000270650700012pt_BR
dc.identifier.doi10.1111/j.1600-0609.2009.01325.xpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsordocumentnumberFAPESP [02/13801-7]pt
dc.date.available2014-11-18T12:36:07Z
dc.date.available2015-11-26T17:50:16Z-
dc.date.accessioned2014-11-18T12:36:07Z
dc.date.accessioned2015-11-26T17:50:16Z-
dc.description.provenanceMade available in DSpace on 2014-11-18T12:36:07Z (GMT). No. of bitstreams: 1 WOS000270650700012.pdf: 93867 bytes, checksum: 7c0293b284d3bbd8fbe9a38a0710f567 (MD5) Previous issue date: 2009en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:50:16Z (GMT). No. of bitstreams: 2 WOS000270650700012.pdf: 93867 bytes, checksum: 7c0293b284d3bbd8fbe9a38a0710f567 (MD5) WOS000270650700012.pdf.txt: 16537 bytes, checksum: aabc8fe228bfc663dc56bfe7c2306688 (MD5) Previous issue date: 2009en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/59857pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/59857
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/59857-
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