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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleIncreased expression and phosphorylation of focal adhesion kinase correlates with dysfunction in the volume-overloaded human heartpt_BR
dc.contributor.authorLopes, MMpt_BR
dc.contributor.authorRibeiro, GCApt_BR
dc.contributor.authorTornatore, TFpt_BR
dc.contributor.authorClemente, CFMZpt_BR
dc.contributor.authorTeiixeira, VPApt_BR
dc.contributor.authorFranchini, KGpt_BR
unicamp.authorUniv Estadual Campinas, Sch Med, Dept Internal Med, BR-13081970 Campinas, SP, Brazil Univ Fed Triangulo Mineiro, Dept Pathol, BR-38025180 Uberaba, Minas Gerais, Brazilpt_BR
dc.subjectfocal adhesion kinase (FAK)pt_BR
dc.subjectheart failurept_BR
dc.subjectmyocardial fibrosispt_BR
dc.subjectsignal transductionpt_BR
dc.subjectvolume overloadpt_BR
dc.subject.wosChronic Mitral Regurgitationpt_BR
dc.subject.wosDesmin-related Cardiomyopathypt_BR
dc.subject.wosCardiac Myocytespt_BR
dc.subject.wosContractile Functionpt_BR
dc.subject.wosInduced Hypertrophypt_BR
dc.description.abstractFAK (focal adhesion kinase) has been shown to mediate the hypertrophic growth of the left ventricle. Experimental results also suggest that FAK may contribute to the structural and functional deterioration of the chronically overloaded left ventricle. In the present study, we postulated that FAK expression and phosphorylation may be altered in the volume-overloaded heart in humans. FAK expression and phosphorylation at Tyr(397) were detected by Western blotting and immunohistochemistry in samples from endomyocardial biopsies from patients with MR (mitral regurgitation; n = 21) and donor subjects (n = 4). Hearts from patients with MR had degenerated cardiac myocytes and areas of fibrosis. In this group, the myocardial collagen area was increased (18 % in MR hearts compared with 3 % in donor hearts respectively) and correlated negatively with left ventricular ejection fraction (r = -0.74; P > 0.001). FAK expression and phosphorylation at Tyr 397 (a marker of the enzyme activity) were increased in samples from MR hearts compared with those from donor hearts (3.1- and 4.9-fold respectively). In myocardial samples from donor hearts, anti-FAK staining was almost exclusively restricted to cardiac myocytes; however, in myocardial samples from MR hearts, staining with the anti-FAK antibody was found to occur in myocytes and the interstitium. There was a positive correlation between collagen and the interstitial areas stained with the anti-FAK antibody (r = 0.76; P > 0.001). Anti-FAK and anti-vimentin staining of the interstitial areas of samples from MR hearts were extensively superimposed, indicating that most of the interstitial FAK was located in fibroblasts. In conclusion, FAK expression and phosphorylation are increased and may contribute to the underlying structural and functional abnormalities in the volume-overloaded heart in
dc.relation.ispartofClinical Sciencept_BR
dc.relation.ispartofabbreviationClin. Sci.pt_BR
dc.publisherPortland Press Ltdpt_BR
dc.identifier.citationClinical Science. Portland Press Ltd, v. 113, n. 41732, n. 195, n. 204, 2007.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.provenanceMade available in DSpace on 2014-07-30T14:33:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2007en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:32:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2007en
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