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Type: Artigo de periódico
Title: Involvement of NO in the failure of neutrophil migration in sepsis induced by Staphylococcus aureus
Author: Crosara-Alberto, DP
Darini, ALC
Inoue, RY
Silva, JS
Ferreira, SH
Cunha, FQ
Abstract: 1. Sepsis induced by S. aureus was used to investigate whether neutrophil migration failure to infectious focus correlates with lethality in Gram-positive bacteria-induced sepsis in mice. 2 By contrast with the sub-lethal (SL-group), the lethal (L-group) intraperitoneal inoculum of S. aureus caused failure of neutrophil migration (92% reduction), high CFU in the exudate, bacteremia and impairment of in vitro neutrophil chemotactic activity. 3 Pre-treatments of L-group with adequate doses of aminoguanidine prevented the neutrophil migration failure and improved the survival of the animals (pre-treated: 43%; untreated: 0% survival). Thus, the impairment of neutrophil migration in the L-group appears to be mediated by nitric oxide (NO). 4 The injection of S. aureus SL-inoculum in iNOS deficient (-/-) or aminoguanidine-treated wild-type mice (pre- and post-treatment), which did not present neutrophil migration failure, paradoxically caused severe peritonitis and high mortality. This fact is explainable by the lack of NO dependent microbicidal activity in migrated neutrophils. 5 In conclusion, although the NO microbicidal mechanism is active in neutrophils, the failure of their migration to the infectious focus may be responsible for the severity and outcome of sepsis.
Subject: nitric oxide
failure of neutrophil migration
S. aureus
Country: Inglaterra
Editor: Nature Publishing Group
Citation: British Journal Of Pharmacology. Nature Publishing Group, v. 136, n. 5, n. 645, n. 658, 2002.
Rights: fechado
Identifier DOI: 10.1038/sj.bjp.0704734
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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