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Type: Artigo de periódico
Title: Detection of Epstein-Barr virus and subsets of lymphoid cells in adenoid tissue of children under 2 years of age
Author: Endo, LH
Vassallo, J
Sakano, E
Brousset, P
Abstract: Epstein-Barr virus (EBV) has been closely associated with undifferenciated nasopharyngeal carcinoma (NPC) and T/ NK nasal non Hodgkin lymphoma. Nevertheless, the presence of EBV in non neoplastic lymphoid tissue of the nasopharynx has been rarely investigated. In a previous study by our group, using in situ hybridization to detect EBV in adenoids of children (2-13 years old) resected because of nasal obstruction due to hypertrophy, we found EBV genome in 72% of the cases. It was now intended to study the frequency of EBV expression in adenoids from children that underwent surgical removal, belonging to a lower age group (1-2 years old). It was also intended to establish which lymphoid subsets are involved in this infection. Adenoidal paraffin sections from 21 patients aged 1-2 years old (mean 1.6 years), 15 males and six females were submitted to double labeling: in situ hybridization with EBER 1/2 probes to detect EBV and immunohistochemistry to determine the lymphocyte typing of EBV-positive cells (CD20 for B-lymphocytes, CD3 for T-lymphocytes and CD56 and CD57 for NK-cells). Among 21 patients, seven showed positive lymphoid cells for EBV (33%). In almost all cases, EBV-positive cells were also CD20-positive. Some EBV-positive cells showed no labeling with any of the lymphoid markers, but in no instance they were positive for CD3, CD56 or CD57. This study confirms the preferential infection of B-lymphocytes by EBV, which in some instances can down regulate the expression of CD20. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Subject: adenoid
Epstein-Barr virus
subsets of lymphoid cells
in situ hybridization
Country: Irlanda
Editor: Elsevier Sci Ireland Ltd
Citation: International Journal Of Pediatric Otorhinolaryngology. Elsevier Sci Ireland Ltd, v. 66, n. 3, n. 223, n. 226, 2002.
Rights: fechado
Identifier DOI: 10.1016/S0165-5876(02)00213-6
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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