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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDifferential regulation of MMP-13 expression in two models of experimentally induced periodontal disease in ratspt_BR
dc.contributor.authorde Aquino, SGpt_BR
dc.contributor.authorGuimaraes, MRpt_BR
dc.contributor.authorStach-Machado, DRpt_BR
dc.contributor.authorda Silva, JAFpt_BR
dc.contributor.authorSpolidorio, LCpt_BR
dc.contributor.authorRossa, Cpt_BR
unicamp.authorde Aquino, Sabrina Garcia Guimaraes, Morgana Rodrigues Rossa, Carlos, Jr. State Univ Sao Paulo UNESP, Sch Dent Araraquara, Dept Diag & Surg, Araraquara, SP, Brazilpt_BR
unicamp.authorSpolidorio, Luis Carlos State Univ Sao Paulo UNESP, Sch Dent Araraquara, Dept Physiol & Pathol, Araraquara, SP, Brazilpt_BR
unicamp.authorStach-Machado, Dagmar Ruth Francisco da Silva, Juliete Aparecida State Univ Campinas UNICAMP, Inst Biol, Dept Microbiol & Immunol, Campinas, SP, Brazilpt_BR
dc.subjectPeriodontal diseasept_BR
dc.subjectExperimental modelspt_BR
dc.subject.wosGingival Crevicular Fluidpt_BR
dc.subject.wosActivated Protein-kinasept_BR
dc.subject.wosMatrix Metalloproteinases Mmpspt_BR
dc.subject.wosCollagenase-3 Mmp-13pt_BR
dc.subject.wosBone Losspt_BR
dc.subject.wosTranscriptional Regulationpt_BR
dc.subject.wosLigament Fibroblastspt_BR
dc.subject.wosAdult Periodontitispt_BR
dc.description.abstractObjective: Evaluate expression of MMP-13 during the course of two models experimentally induced periodontal disease in rats. Design: Expression of MMP-13 at mRNA and protein levels was studied, respectively, by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Two experimental models were used: LPS injections and ligature placement. 30 jig of LPS from Eschericia coli was injected twice a week into the palatal aspect of upper molars. Ligatures were placed at the gingival margin around lower first molars. Controls received injections of PBS vehicle and no ligatures on lower molars. Samples were collected 5,15 and 30 days after initiation of periodontal disease and processed for extraction of total RNA, total protein, and routinely processed for histology. Results: Both experimental models produced a significant increase on the inflammatory infiltrate that paralleled elevated levels of MMP-13 mRNA and protein at 5 and 15 days. The LPS model was associated with a sustained level of inflammation and increased MMP-13 mRNA throughout the 30 days, whereas the ligature model showed a decrease on the severity of inflammation and MMP-13 mRNA at the 30-day period. interestingly, MMP-13 protein levels were diametrically contrary to the mRNA levels. Conclusion: MMP-13 expression during LPS- and ligature-induced experimental periodontal disease follows the increase on severity of inflammation at the earliest periods. At 30 days, there is a decrease on the severity of inflammation on the ligature model associated with decreased MMP-13 mRNA. There is a lack of transcription-translation coupling of MMP-13 gene in both experimental models. (C) 2009 Elsevier Ltd. All rights
dc.relation.ispartofArchives Of Oral Biologypt_BR
dc.relation.ispartofabbreviationArch. Oral Biol.pt_BR
dc.publisherPergamon-elsevier Science Ltdpt_BR
dc.identifier.citationArchives Of Oral Biology. Pergamon-elsevier Science Ltd, v. 54, n. 7, n. 609, n. 617, 2009.pt_BR
dc.sourceWeb of Sciencept_BR
unicamp.cruespUNESP USPpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipState of Sao Paulo, Brazil [2005/04428-9, 2006/07283-4]pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsordocumentnumberFAPESP [2005/04428-9, 2006/07283-4]pt
dc.description.sponsordocumentnumberState of Sao Paulo, Brazil [2005/04428-9, 2006/07283-4]pt
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:05:27Z (GMT). No. of bitstreams: 2 WOS000267480800001.pdf: 1002446 bytes, checksum: 9442b9168aa552d50c39440c0c634e77 (MD5) WOS000267480800001.pdf.txt: 39071 bytes, checksum: 564b3a003a8399f820b5243f01eae0f4 (MD5) Previous issue date: 2009en
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