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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleCharacterization of the screwworm flies Cochliomyia hominivorax and Cochliomyia macellaria by PCR-RFLP of mitochondrial DNApt_BR
dc.contributor.authorLitjens, Ppt_BR
dc.contributor.authorLessinger, ACpt_BR
dc.contributor.authorDe Azeredo-Espin, AMLpt_BR
unicamp.authorUniv Estadual Campinas, UNICAMP, CBMEG, Lab Genet Anim, BR-13083970 Campinas, SP, Brazil Univ Estadual Campinas, Dept Genet & Evolucao, Sao Paulo, Brazilpt_BR
dc.subjectCochliomyia hominivoraxpt_BR
dc.subjectCochliomyia macellariapt_BR
dc.subjectmolecular identificationpt_BR
dc.subjectmolecular markerspt_BR
dc.subjectmtDNApt_BR
dc.subjectPCR-RFLPpt_BR
dc.subjectscrewwormpt_BR
dc.subjectBrazilpt_BR
dc.subject.wosPolymerase Chain-reactionpt_BR
dc.subject.wosFragment-length-polymorphismpt_BR
dc.subject.wosNew-world Screwwormpt_BR
dc.subject.wosNorth-africapt_BR
dc.subject.wosDipterapt_BR
dc.subject.wosCalliphoridaept_BR
dc.subject.wosFlypt_BR
dc.subject.wosIdentificationpt_BR
dc.subject.wosColeopterapt_BR
dc.subject.wosPopulationspt_BR
dc.description.abstractThe primary screwworm fly Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) is one of the most important insect pests of livestock in neotropical regions, whereas Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), the secondary screwworm, is of medical and sanitary importance because of its role in the dissemination of pathogens. These two species share morphological similarities and both may occur in the same myiasis, but in different developmental stages. In this work, the usefulness of PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) of mitochondrial DNA (mtDNA) for the unambiguous identification of C. hominivorax and C. macellaria was investigated. Two specific regions of mtDNA were amplified: 870bp from Cytochrome oxidase subunit I and 2100bp from the A+T rich/12S region from C. hominivorax and C. macellaria specimens from different areas of Brazil. Reliable species-specific PCR-RFLP results were obtained for the CO I region and the A+T rich/12S region using the restriction enzymes Dra I and Ssp I. These results confirm the conservation of CO I diagnostic restriction sites previously reported and demonstrate the usefulness of the control region sequences as an efficient marker for PCR-RFLP identification of Brazilian screwworm flies. The occurrences of intraspecific polymorphic patterns are discussed based on frequencies and potential conflicts for species identification. PCR-RFLP provides a potentially useful method for identifying samples from the areas where these species are monitored.pt
dc.relation.ispartofMedical And Veterinary Entomologypt_BR
dc.relation.ispartofabbreviationMed. Vet. Entomol.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherBlackwell Science Ltdpt_BR
dc.date.issued2001pt_BR
dc.date.monthofcirculationJUNpt_BR
dc.identifier.citationMedical And Veterinary Entomology. Blackwell Science Ltd, v. 15, n. 2, n. 183, n. 188, 2001.pt_BR
dc.language.isoenpt_BR
dc.description.volume15pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage183pt_BR
dc.description.lastpage188pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0269-283Xpt_BR
dc.identifier.wosidWOS:000169497200009pt_BR
dc.identifier.doi10.1046/j.1365-2915.2001.00289.xpt_BR
dc.date.available2014-11-18T12:43:04Z
dc.date.available2015-11-26T16:54:54Z-
dc.date.accessioned2014-11-18T12:43:04Z
dc.date.accessioned2015-11-26T16:54:54Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:54:54Z (GMT). No. of bitstreams: 2 WOS000169497200009.pdf: 102077 bytes, checksum: e12f52de4996cbcac6a940fa2ce98e0d (MD5) WOS000169497200009.pdf.txt: 26160 bytes, checksum: 0fbd2b7768ec77c47cb13784a1527564 (MD5) Previous issue date: 2001en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62913pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/62913
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62913-
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