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Type: Artigo de periódico
Title: Effect of Bcl-2 overexpression on mitochondrial structure and function
Author: Kowaltowski, AJ
Cosso, RG
Campos, CB
Fiskum, G
Abstract: Overexpression of the antiapoptotic Bcl-2 protein enhances the uptake of fluorimetric dyes sensitive to mitochondrial membrane potential, suggesting that Bcl-2 changes the mitochondrial proton gradient. In this study, we performed calibrated measurements of mitochondrial respiration, membrane potential, DeltapH, and intramitochondrial [K+] in digitonin-permeabilized PC12 and GT1-7 neural cells that either do not express human Bcl-2 (control transfectants) or that were transfected with and overexpressed the human bcl-2 gene to evaluate whether Bcl-2 alters mitochondrial inner membrane ion transport. We found that although Bcl-2-overexpressing cells exhibit higher fluorescence responses to membrane potential, pH, and K+-sensitive dyes, this increased response is due to an enhanced accumulation of these dyes and not an increased mitochondrial membrane potential, DeltapH, or [K+]. This result is supported by the presence of equal respiratory rates in Bcl-2+ and Bcl-2- cells. Possible structural alterations in Bcl-2+ mitochondria that could account for increases in fluorescent dye uptake were evaluated using flow cytometry particle sizing and light scattering determinations. These experiments established that Bcl-2-overexpressing mitochondria present both increased volume and structural complexity. We suggest that increased mitochondrial volume and structural complexity in Bcl-2+ cells may be related to many of the effects of this protein involved in the prevention of cell death.
Country: EUA
Editor: Amer Soc Biochemistry Molecular Biology Inc
Citation: Journal Of Biological Chemistry. Amer Soc Biochemistry Molecular Biology Inc, v. 277, n. 45, n. 42802, n. 42807, 2002.
Rights: fechado
Identifier DOI: 10.1074/jbc.M207765200
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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