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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEvidence for increased expression of tissue factor and protease-activated receptor-1 in human esophageal cancerpt_BR
dc.contributor.authorRibeiro, FSpt_BR
dc.contributor.authorSimao, TApt_BR
dc.contributor.authorAmoedo, NDpt_BR
dc.contributor.authorAndreollo, NApt_BR
dc.contributor.authorLopes, LRpt_BR
dc.contributor.authorAcatauass, Rpt_BR
dc.contributor.authorRumjanek, FDpt_BR
dc.contributor.authorAlbano, RMpt_BR
dc.contributor.authorPinto, LFRpt_BR
dc.contributor.authorMonteiro, RQpt_BR
unicamp.author.emailrobsonqm@bioqmed.ufrj.brpt_BR
unicamp.authorRibeiro, Fabiana S. Simao, Tatiana A. Albano, Rodolpho M. Pinto, Luis Felipe R. Univ Estado Rio de Janeiro, Dept Bioquim, IBRAG, Rio De Janeiro, Brazilpt_BR
unicamp.authorRibeiro, Fabiana S. Amoedo, Nivea D. Rumjanek, Franklin D. Monteiro, Robson Q. Univ Fed Rio de Janeiro, Inst Bioquim Med, BR-21941590 Rio De Janeiro, Brazilpt_BR
unicamp.authorAndreollo, Nelson A. Lopes, Luiz Roberto Univ Estadual Campinas, Dept Cirugia & Gastroctr, Fac Ciencias Med, BR-13081970 Campinas, SP, Brazilpt_BR
unicamp.authorAcatauass, Rodolfo Univ Estado Rio de Janeiro, Hosp Univ Pedro Ernesto, Serv Cirurgia Torac, Rio De Janeiro, Brazilpt_BR
dc.subjectesophageal squamous cell carcinomapt_BR
dc.subjectclotting initiator tissue factorpt_BR
dc.subjectprotease activated receptor-1pt_BR
dc.subjectinterleukin-8pt_BR
dc.subject.wosSquamous-cell Carcinomapt_BR
dc.subject.wosHuman Pancreatic-cancerpt_BR
dc.subject.wosVegf-c Expressionpt_BR
dc.subject.wosTumor Angiogenesispt_BR
dc.subject.wosBlood-coagulationpt_BR
dc.subject.wosInterleukin-8pt_BR
dc.subject.wosMetastasispt_BR
dc.subject.wosMechanismspt_BR
dc.subject.wosInductionpt_BR
dc.subject.wosInvasionpt_BR
dc.description.abstractIt has been suggested that the blood clotting initiator protein, tissue factor (TF), participates in tumor growth, metastasis and angiogenesis. In addition, a family of G protein-coupled-receptors known as protease-activated receptors (PARs) has also been implicated in tumor biology. These receptors might be activated by blood coagulation proteases thus eliciting a number of pro-tumoral responses, including the expression of interleukin-8 (IL-8). Therefore, in this study we analyzed the expression of TF, PAR-1, PAR-2 and IL-8 genes in patients with esophageal cancer, one of the most aggressive neoplastic diseases. Total RNA was extracted from tissue samples (tumor and the corresponding normal mucosa) obtained from patients submitted to esophagectomy or endoscopy and further analyzed by semi-quantitative reverse transcriptase-polymerase (RT-PCR) and/or real-time quantitative PCR (qPCR). Expression of full-length transmembrane TF was significantly higher in tumor samples whereas no differences were observed in alternatively spliced TF transcripts. Tumor tissue showed increased mRNA levels for PAR-1 but not PAR-2. Remarkably, IL-8 expression was not detected in most normal tissues but showed very high expression in tumor samples. As expected, qPCR revealed greater differences in the expression pattern of all transcripts analyzed but the general profile was very similar to that observed by RT-PCR. Altogether our data suggest a possible role for blood clotting proteins in the biology of human esophageal cancer.pt
dc.description.noteO TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE FEVEREIRO DE 2015.pt
dc.relation.ispartofOncology Reportspt_BR
dc.relation.ispartofabbreviationOncol. Rep.pt_BR
dc.publisher.cityAthenspt_BR
dc.publisher.countryGréciapt_BR
dc.publisherSpandidos Publ Ltdpt_BR
dc.date.issued2009pt_BR
dc.date.monthofcirculationJUNpt_BR
dc.identifier.citationOncology Reports. Spandidos Publ Ltd, v. 21, n. 6, n. 1599, n. 1604, 2009.pt_BR
dc.language.isoenpt_BR
dc.description.volume21pt_BR
dc.description.issuenumber6pt_BR
dc.description.firstpage1599pt_BR
dc.description.lastpage1604pt_BR
dc.rightsembargopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1021-335Xpt_BR
dc.identifier.wosidWOS:000266185900033pt_BR
dc.identifier.doi10.3892/or_00000393pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.date.available2014-11-17T08:45:10Z
dc.date.available2015-11-26T16:41:39Z-
dc.date.accessioned2014-11-17T08:45:10Z
dc.date.accessioned2015-11-26T16:41:39Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:41:39Z (GMT). No. of bitstreams: 2 WOS000266185900033.pdf: 168087 bytes, checksum: 4399b6ba5ca9e8d90479991f8cd9374b (MD5) WOS000266185900033.pdf.txt: 29226 bytes, checksum: f050b541347977cd6595cbb43fcb73a5 (MD5) Previous issue date: 2009en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66059pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/66059
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66059-
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