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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleExpression and distribution of NOS1 and NOS3 in the myocardium of angiotensin II-infused ratspt_BR
dc.contributor.authorTambascia, RCpt_BR
dc.contributor.authorFonseca, PMpt_BR
dc.contributor.authorCorat, PDCpt_BR
dc.contributor.authorMoreno, Hpt_BR
dc.contributor.authorSaad, MJApt_BR
dc.contributor.authorFranchini, KGpt_BR
unicamp.authorState Univ Campinas, UNICAMP, Sch Med, Dept Internal Med, Campinas, SP, Brazilpt_BR
dc.subjectangiotensin IIpt_BR
dc.subjectnitric oxidept_BR
dc.subjectnitric oxide synthasept_BR
dc.subjectheartpt_BR
dc.subjectmyocardiumpt_BR
dc.subject.wosNitric-oxide Synthasept_BR
dc.subject.wosSmooth-muscle Cellspt_BR
dc.subject.wosEndothelial-cellspt_BR
dc.subject.wosCardiac Myocytespt_BR
dc.subject.wosHypertrophypt_BR
dc.subject.wosFibroblastspt_BR
dc.subject.wosActivationpt_BR
dc.subject.wosParacrinept_BR
dc.subject.wosIsoformpt_BR
dc.subject.wosSystempt_BR
dc.description.abstractStudies have indicated a complex functional interaction between angiotensin (Ang) II. and NO in the heart. The purpose of the present study was to examine the protein expression and tissue distribution of NO synthases 1 (NOS1) and 3 (NOS3) in the myocardium of rats that underwent continuous infusion of Ang II. at 2 different rates (10 and 40 ng . kg(-1) . min(-1)) for 6 days. Mean arterial pressure increased by approximate to 15 mm Hg in rats infused with Ang II at 40 ng . kg(-1) . min(-1), but it remained close to the values observed in saline-infused rats (approximate to 110 mm Hg) when Ang II was infused at 10 ng . kg(-1) . min(-1). The protein expression of a 160-kDa NOS1 and a 135-kDa NOS3 were found to increase (approximate to 200%) in the myocardium of rats infused with both subpressor and presser doses of Ang II. Immunohistochemistry studies showed that NOS1 and NOS3 are differentially expressed in myocardial cells. NOS1 was detected in cardiac myocytes and in smooth muscle cells of small and large coronary arteries, whereas NOS3 was detected in the endothelium and in perivascular and interstitial tissues, but NOS3 was not detected in cardiac or smooth muscle cells. Ang II infusion enhanced the tissue immunoreactivity of both isoforms in their specific locations but did not change the distribution throughout the myocardium. Myocardium staining with anti-angiotensin type 1 (AT(1)) receptor antibody indicated that AT(1) receptor is expressed in cardiac myocytes, coronary smooth muscle cells, and interstitial and perivascular tissues. Ang II infusion did not change the protein expression and distribution of AT(1) receptor in the myocardium, These results indicate that long-term increases in the circulating levels of Ang II modulate the protein expression of NOS1 and NOS3 and, consequently, the function of the local myocardial NO system.pt
dc.relation.ispartofHypertensionpt_BR
dc.relation.ispartofabbreviationHypertensionpt_BR
dc.publisher.cityPhiladelphiapt_BR
dc.publisher.countryEUApt_BR
dc.publisherLippincott Williams & Wilkinspt_BR
dc.date.issued2001pt_BR
dc.date.monthofcirculationJUNpt_BR
dc.identifier.citationHypertension. Lippincott Williams & Wilkins, v. 37, n. 6, n. 1423, n. 1428, 2001.pt_BR
dc.language.isoenpt_BR
dc.description.volume37pt_BR
dc.description.issuenumber6pt_BR
dc.description.firstpage1423pt_BR
dc.description.lastpage1428pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0194-911Xpt_BR
dc.identifier.wosidWOS:000169428000014pt_BR
dc.date.available2014-11-18T03:00:35Z
dc.date.available2015-11-26T17:45:37Z-
dc.date.accessioned2014-11-18T03:00:35Z
dc.date.accessioned2015-11-26T17:45:37Z-
dc.description.provenanceMade available in DSpace on 2014-11-18T03:00:35Z (GMT). No. of bitstreams: 1 WOS000169428000014.pdf: 2268753 bytes, checksum: e69d6b3ac33039f56f9dee473d1c1976 (MD5) Previous issue date: 2001en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:45:37Z (GMT). No. of bitstreams: 2 WOS000169428000014.pdf: 2268753 bytes, checksum: e69d6b3ac33039f56f9dee473d1c1976 (MD5) WOS000169428000014.pdf.txt: 28905 bytes, checksum: d5b868348538c90ac564d2fe80b41946 (MD5) Previous issue date: 2001en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66450pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/66450
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66450-
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