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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleIdentification of Stylosanthes guianensis varieties using molecular genetic analysispt_BR
dc.contributor.authorSantos-Garcia, MOpt_BR
dc.contributor.authorKaria, CTpt_BR
dc.contributor.authorResende, RMSpt_BR
dc.contributor.authorChiari, Lpt_BR
dc.contributor.authorVieira, MLCpt_BR
dc.contributor.authorZucchi, MIpt_BR
dc.contributor.authorSouza, APpt_BR
unicamp.authorSantos-Garcia, M. O. Souza, A. P. Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorKaria, C. T. Embrapa Cerrados, BR-73310970 Planaltina, DF, Brazilpt_BR
unicamp.authorResende, R. M. S. Chiari, L. Embrapa Gado Corte, BR-79002970 Campo Grande, MS, Brazilpt_BR
unicamp.authorVieira, M. L. C. Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba, SP, Brazilpt_BR
unicamp.authorZucchi, M. I. Polo Reg Ctr APTA, BR-13400970 Piracicaba, SP, Brazilpt_BR
unicamp.authorSouza, A. P. Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subject.wosMicrosatellite Markerspt_BR
dc.subject.wosSpecies Complexpt_BR
dc.description.abstractBackground and aims The botanical classification of Stylosanthes guianensis is controversial, and few studies have used molecular markers to analyse this species. We used microsatellite markers to study the genetic diversity and population structure of S. guianensis and compare our results with the current infraspecific botanical classification. Methodology A representative sample from the S. guianensis Brazilian germplasm collection (150 accessions) was analysed using 20 microsatellite loci. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign accessions into clusters. A dendrogram was constructed based on Roger's genetic distances. Principal results The number of alleles per locus varied from 2 to 11, with an average of 4.7. The observed (H-O) and expected (H-E) heterozygosity values varied from 0 to 0.58 (mean of 0.18) and from 0.04 to 0.83 (mean of 0.55), respectively. Nine groups were assembled in STRUCTURE, and these groups were consistent with clusters inferred from the genetic distances and taxonomic varieties described for S. guianensis. The G(ST) among the nine groups was 0.46. Conclusions The low HO and the G(ST) values observed are in agreement with the outcrossing rate (26 %) estimated for this species. The data indicate a high genetic diversity among and within the botanical varieties and suggest that microsatellite-based information can be combined with classical taxonomy to elucidate infraspecific
dc.relation.ispartofAob Plantspt_BR
dc.relation.ispartofabbreviationAob Plantspt_BR
dc.publisherOxford Univ Presspt_BR
dc.identifier.citationAob Plants. Oxford Univ Press, 2012.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [2005/51010-0]pt
dc.description.sponsordocumentnumberFAPESP [2005/52211-9]pt
dc.description.provenanceMade available in DSpace on 2014-07-30T18:00:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
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