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Type: Artigo de periódico
Title: Opening of the mitochondrial permeability transition pore by uncoupling or inorganic phosphate in the presence of Ca2+ is dependent on mitochondrial-generated reactive oxygen species
Author: Kowaltowski, AJ
Castilho, RF
Vercesi, AE
Abstract: In this study, we show that mitochondrial membrane permeability transition in Ca2+-loaded mitochondria treated with carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) or inorganic phosphate (P-i) is preceded by enhanced production of H2O2. This production is inhibited either by ethylene glycol-bis(b-aminoethyl ether)N,N,N',N'-tetraacetic acid (EGTA) or Mg2+, but not by cyclosporin A, Permeability transition is prevented either by EGTA, catalase or dithiothreitol, suggesting the involvement of Ca2+, H2O2 and oxidation of membrane protein thiols in this mechanism, When mitochondria are incubated under anaerobiosis, no permeabilization or H2O2 production occurs, Based on these results we conclude that mitochondrial permeability transition induced by P-i or FCCP-uncoupling is dependent on mitochondrial-generated reactive oxygen species.
Subject: mitochondrial permeability transition pore
reactive oxygen species
Country: Holanda
Editor: Elsevier Science Bv
Citation: Febs Letters. Elsevier Science Bv, v. 378, n. 2, n. 150, n. 152, 1996.
Rights: fechado
Identifier DOI: 10.1016/0014-5793(95)01449-7
Date Issue: 1996
Appears in Collections:Unicamp - Artigos e Outros Documentos

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