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Type: Artigo de periódico
Title: Polymorphic microsatellite loci for Stylosanthes macrocephala Ferr. et Costa, a tropical forage legume
Author: Santos, MO
Sassaki, RP
Ferreira, THS
Resende, RMS
Chiari, L
Karia, CT
Faleiro, FG
Jungmann, L
Zucchi, MI
Souza, AP
Abstract: Microsatellites were developed for Stylosanthes macrocephala, aiming at developing tools for studying the genetic diversity of this species. A total of 13 polymorphic microsatellite markers were isolated from a S. macrocephala enriched genomic library. The isolated microsatellites were characterized in 20 accessions of the S. macrocephala germplasm collection belonging to the Empresa Brasileira de Pesquisa Agropecuaria (Embrapa) Cerrados. The number of alleles per locus varied from 2 to 11, with an average of 4 alleles per locus. The observed and expected heterozygosities ranged from 0 to 0.25 and 0.05 to 0.90, respectively. Cross-amplification of the S. macrocephala polymorphic microsatellites was evaluated in three other Stylosanthes species. The microsatellites reported herein are the first set of microsatellite markers developed for S. macrocephala and are potentially useful for further studies on genetic diversity, conservation and breeding of this species.
Subject: Molecular markers
Genetic diversity
Genomic enriched library
Country: Holanda
Editor: Springer
Citation: Conservation Genetics Resources. Springer, v. 1, n. 1, n. 481, n. 485, 2009.
Rights: fechado
Identifier DOI: 10.1007/s12686-009-9112-x
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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