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Type: Artigo de periódico
Title: Role of Fe(III) in Fe(II)citrate-mediated peroxidation of mitochondrial membrane lipids
Author: Castilho, RF
Meinicke, AR
Vercesi, AE
Hermes-Lima, M
Abstract: In this report we study the effect of Fe(III) on lipid peroxidation induced by Fe(II)citrate in mitochondrial membranes, as assessed by the production of thiobarbituric acid-reactive substances and antimycin A-insensitive oxygen uptake. The presence of Fe(III) stimulates initiation of lipid peroxidation when low citrate:Fe(II) ratios are used (less than or equal to 4:1). For a citrate:total iron ratio of 1:1 the maximal stimulation of lipid peroxidation by Fe(III) was observed when the Fe(II):Fe(III) ratio was in the range of 1:1 to 1:2. The lag phase that accompanies oxygen uptake was greatly diminished by increasing concentrations of Fe(III) when the citrate:total iron ratio was 1:1, but not when this ratio was higher. It is concluded that the increase of lipid peroxidation by Fe(III) is observed only when low citrate:Fe(II) ratios were used. Similar results were obtained using ATP as a ligand of iron. Monitoring the rate of spontaneous Fe(II) oxidation by measuring oxygen uptake in buffered medium, in the absence of mitochondria, Fe(III)-stimulated oxygen consumption was observed only when a low citrate:Fe(II) ratio was used. This result suggests that Fe(III) may facilitate the initiation and/or propagation of lipid peroxidation by increasing the rate of Fe(II)citrate-generated reactive oxygen species.
Subject: Fe(II)citrate
free radicals
lipid peroxidation
reactive oxygen species
Country: Holanda
Editor: Kluwer Academic Publ
Citation: Molecular And Cellular Biochemistry. Kluwer Academic Publ, v. 196, n. 41671, n. 163, n. 168, 1999.
Rights: fechado
Identifier DOI: 10.1023/A:1006988129221
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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