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Type: Artigo de periódico
Title: Validation of a method for simultaneous quantification of total carotenes and tocols in vegetable oils by HPLC
Author: Silva, SM
Rocco, SA
Sampaio, KA
Taham, T
da Silva, LHM
Ceriani, R
Meirelles, AJA
Abstract: A normal-phase HPLC method for analysis of carotenes, tocopherols and tocotrienols has been developed and validated. In this work we presented a modification to the official AOCS method for analysis of tocols which allowed simultaneous quantification of the three groups of compounds, including carotenes. Analytes were separated using a gradient mobile phase (hexane and isopropanol) and with a gradient flow rate (1-2 mL min(-1)). The column effluent was monitored by Photo Diode Array detector (PDA) set at 292 nm (tocols) and 455 nm (beta-carotene) and by fluorescence detector set at an excitation wavelength of 290 and 330 nm emission. Inter- and intra-run accuracies and precision of the analytical method were better than +/- 15%. The lower limit of quantification was 5.0 mg L(-1) for the tocols and 0.1 mg L(-1) for carotenes. The method has been applied for the quantification of these compounds in Amazon oils. (C) 2011 Elsevier Ltd. All rights reserved.
Subject: Amazon oils
Country: Inglaterra
Editor: Elsevier Sci Ltd
Citation: Food Chemistry. Elsevier Sci Ltd, v. 129, n. 4, n. 1874, n. 1881, 2011.
Rights: fechado
Identifier DOI: 10.1016/j.foodchem.2011.05.137
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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