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|Type:||Artigo de periódico|
|Title:||Use of chemically modified silica with beta-diketoamine groups for separation of alpha-lactoalbumin from bovine milk whey by affinity chromatography|
|Abstract:||Silica gel surface was chemically modified with beta-diketoamine groups by reacting the silanol from the silica surface with 3-aminopropyl-triethoxysilane and 3-bromopentanedione, With this material, copper ions were adsorbed from aqueous solutions, The chemical analysis of the silica-gel-immobilized acetylacetone provided a quantity of 0.67 mmol g(-1) of organic groups attached to the support and 0.63 mmol g(-1) of copper, This material was used as a stationary phase in IMAC (immobilized metal affinity chromatography), to separate alpha-lactoalbumin from bovine milk whey, The results showed an efficient separation in the chromatographic column, The possibility of reutilization of the stationary phase was also investigated. (C) 1997 Academic Press|
|Editor:||Academic Press Inc Jnl-comp Subscriptions|
|Citation:||Journal Of Colloid And Interface Science. Academic Press Inc Jnl-comp Subscriptions, v. 185, n. 2, n. 313, n. 316, 1997.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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