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Type: Artigo de periódico
Title: Suramin inhibits respiration and induces membrane permeability transition in isolated rat liver mitochondria
Author: Bernardes, CF
Fagian, MM
Meyer-Fernandes, JR
Castilho, RF
Vercesi, AE
Abstract: Suramin, a polysulfonated naphthylamine. caused a dose dependent inhibition of carbonyl cyanide p-(trifluoromethoxy)phenyihydrazone-stimulated respiration supported either by succinate or a cocktail of alpha ketoglutarate, malate and isocitrate in isolated rat liver mitochondria. The half-maximum effect was obtained at 40 and 140 muM suramin for NADH- or FADH(2)-linked substrates, respectively. The respiration supported by N,N,,N'N'-tetramethyl-p-phenylenediamine oxidation was unaffected by suramin (less than or equal to 500 muM). The latter respiratory substrate was used in the studies at examining the effects of suramin on the permeability properties of the inner mitochondrial membrane. These experiments provided evidence that suramin causes Ca2+-dependent mitochondrial swelling sensitive to the mitochondrial permeability transition (MPT) inhibitors cyclosporin A, ADP and Mg2+. In addition, suramin decreased the content of reduced mitochondrial membrane protein thiols suggesting that thiol oxidation is the mechanism underlying suramin-induced MPT. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
Subject: suramin
isolated rat liver mitochondria
mitochondrial membrane permeability transition
thiol oxidation
Country: Irlanda
Editor: Elsevier Sci Ireland Ltd
Citation: Toxicology. Elsevier Sci Ireland Ltd, v. 169, n. 1, n. 17, n. 23, 2001.
Rights: fechado
Identifier DOI: 10.1016/S0300-483X(01)00477-2
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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