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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleAdhesion of bovine enterotoxigenic Escherichia coli (ETEC) by type 1-like fimbriaept_BR
dc.contributor.authorCatani, CFpt_BR
dc.contributor.authorYamada, ATpt_BR
dc.contributor.authorVidotto, MCpt_BR
dc.contributor.authorYano, Tpt_BR
unicamp.authorUNIV ESTADUAL CAMPINAS,DEPT MICROBIOL & IMMUNOL,CAMPINAS,SP,BRAZIL UNIV ESTADUAL CAMPINAS,DEPT HIST & EMBRIOL,CAMPINAS,SP,BRAZIL UNIV ESTADUAL LONDRINA,DEPT PATOL GERAL,BR-86051 LONDRINA,PR,BRAZILpt_BR
dc.subjectfimbriaept_BR
dc.subjectETECpt_BR
dc.subjectEscherichia colipt_BR
dc.subjectMSHApt_BR
dc.subject.wosColonization Factorpt_BR
dc.subject.wosSmall-intestinept_BR
dc.subject.wosAntigenpt_BR
dc.subject.wosHemagglutinationpt_BR
dc.subject.wosPurificationpt_BR
dc.subject.wosPilipt_BR
dc.subject.wosErythrocytespt_BR
dc.subject.wosDiarrheapt_BR
dc.subject.wosProteinspt_BR
dc.subject.wosMannosept_BR
dc.description.abstractEnterotoxigenic Escherichia coli (STa+) strains were isolated from adult bovine with diarrhea. These strains did not express any known ETEC specific adhesins. Although hemagglutination with rat and sheep erythrocytes was observed in the presence of D-mannose (MRHA), these strains also showed mannose-sensitive hemagglutination (MSHA) with guinea-pig erythrocytes. Electron microscopic studies revealed the presence of fimbria-like structures (provisionally called 'F43ms') on bacterial cells grown at 37 degrees C but not on cells grown at 18 degrees C, However, it was observed by SDS-PAGE that the J-1 strain (F43ms(+)) produces a protein similar to F1 fimbriae, and this strain hybridized with a DNA probe for F1 fimbriae, Immunogold-labelling techniques indicated that a rabbit anti-serum is specific for F43ms fimbrial structures, but not for Type 1 fimbriae. The immunofluorescence test carried out with semipurified F43ms on bovine brush borders suggests that the fimbria-like structures are responsible for the adhesion to bovine epithelial cells.pt
dc.relation.ispartofFems Microbiology Letterspt_BR
dc.relation.ispartofabbreviationFEMS Microbiol. Lett.pt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryHolandapt_BR
dc.publisherElsevier Science Bvpt_BR
dc.date.issued1996pt_BR
dc.date.monthofcirculationAPR 1pt_BR
dc.identifier.citationFems Microbiology Letters. Elsevier Science Bv, v. 137, n. 41700, n. 241, n. 245, 1996.pt_BR
dc.language.isoenpt_BR
dc.description.volume137pt_BR
dc.description.issuenumber41700pt_BR
dc.description.firstpage241pt_BR
dc.description.lastpage245pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0378-1097pt_BR
dc.identifier.wosidWOS:A1996UM10800020pt_BR
dc.date.available2014-12-02T16:31:03Z
dc.date.available2015-11-26T17:41:30Z-
dc.date.accessioned2014-12-02T16:31:03Z
dc.date.accessioned2015-11-26T17:41:30Z-
dc.description.provenanceMade available in DSpace on 2014-12-02T16:31:03Z (GMT). No. of bitstreams: 1 WOSA1996UM10800020.pdf: 554591 bytes, checksum: 675136c8648e3f5fe761da842763d054 (MD5) Previous issue date: 1996en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:41:30Z (GMT). No. of bitstreams: 2 WOSA1996UM10800020.pdf: 554591 bytes, checksum: 675136c8648e3f5fe761da842763d054 (MD5) WOSA1996UM10800020.pdf.txt: 16740 bytes, checksum: 686e2ade87b984a86e0fc73d49ec4573 (MD5) Previous issue date: 1996en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76768pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/76768
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76768-
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