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Type: Artigo de periódico
Title: Catalases and thioredoxin peroxidase protect Saccharomyces cerevisiae against Ca2+-induced mitochondrial membrane permeabilization and cell death
Author: Kowaltowski, AJ
Vercesi, AE
Rhee, SG
Netto, LES
Abstract: The involvement of reactive oxygen species in Ca2+-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2,4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae cells were very resistant to Ca2+ and inorganic phosphate or t-butyl hydroperoxide-induced mitochondrial membrane permeabilization, but suffered an immediate decrease in mitochondrial membrane potential when treated with Ca2+ and the dithiol binding reagent phenylarsine oxide. In contrast, S, cerevisiae spheroblasts lacking the TPx gene and/or treated with ATZ suffered a decrease in mitochondrial membrane potential, generated higher amounts of hydrogen peroxide and had decreased viability under these conditions. In all cases, the decrease in mitochondrial membrane potential could be inhibited by ethylene glycol-bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid, dithiothreitol or ADP, but not by cyclosporin A. We conclude that TPx and catalase act together, maintaining cell viability and protecting S, cerevisiae mitochondria against Ca2+-promoted membrane permeabilization, which presents similar characteristics to mammalian permeability transition. (C) 2000 Federation of European Biochemical Societies.
Subject: mitochondrion
mitochondriai permeability transition
cell death
Country: Holanda
Editor: Elsevier Science Bv
Citation: Febs Letters. Elsevier Science Bv, v. 473, n. 2, n. 177, n. 182, 2000.
Rights: fechado
Identifier DOI: 10.1016/S0014-5793(00)01526-X
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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