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Type: Artigo de periódico
Title: Preliminary X-ray diffraction studies of rabbit muscle triose phosphate isomerase (TIM)
Author: Aparicio, R
Ferreira, ST
Leite, NR
Polikarpov, I
Abstract: Triose phosphate isomerase (TIM) is responsible for the interconversion between GAP and DHAP in the glycolytic pathway. Two crystal forms belonging to space group P2(1)2(1)2(1) were obtained by the hanging-drop method and were designated A and B. Synchrotron X-ray diffraction data were collected for both forms. Form A had unit-cell parameters a = 65.14, b = 72.45, c = 93.24 Angstrom and diffracted to 2.25 Angstrom at 85 K, whereas form B had unit-cell parameters a = 73.02, b = 79.80, c = 172.85 Angstrom and diffracted to 2.85 Angstrom at room temperature. Molecular replacement was employed to solve the structures, using human TIM as a search model. Further refinement of both structures is under way and is expected to shed light on the recently reported conformational studies for rabbit TIM.
Country: Dinamarca
Editor: Munksgaard Int Publ Ltd
Citation: Acta Crystallographica Section D-biological Crystallography. Munksgaard Int Publ Ltd, v. 56, n. 1492, n. 1494, 2000.
Rights: aberto
Identifier DOI: 10.1107/S0907444900011380
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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