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Type: Artigo de periódico
Title: N-15-labed glycine synthesis
Author: Tavares, CRO
Bendassolli, JA
Coelho, F
Sant'ana, CR
Prestes, CV
Abstract: This work describes a method for N-15-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia ((NH3)-N-15). Special care was taken to avoid possible (NH3)-N-15 losses, since its production cost is high. In that respect, although the purchase cost of the N-13-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable (NH3)-N-15 (aq) volumes in the reaction were used (50, 100, and 150 mL), in order to calibrate the best operational condition; glycine masses obtained were 1.7, 2, and 3.2 g, respectively. With the development of a system for (NH3)-N-15 recovery, it was possible to recover 71, 83, and 87% of the ammonia initially used in the synthesis. With the required adaptations, the same system was used to recover methanol, and 75% of the methanol initially used in the amino acid purification process were recovered.
Subject: stable isotope
amino acid
Country: Brasil
Editor: Acad Brasileira De Ciencias
Citation: Anais Da Academia Brasileira De Ciencias. Acad Brasileira De Ciencias, v. 78, n. 3, n. 441, n. 449, 2006.
Rights: aberto
Identifier DOI: 10.1590/S0001-37652006000300005
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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