Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/86099
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleLeaf-, Panel- And Latex-expressed Sequenced Tags From The Rubber Tree (hevea Brasiliensis) Under Cold-stressed And Suboptimal Growing Conditions: The Development Of Gene-targeted Functional Markers For Stress Responsept_BR
dc.contributor.authorSilva C.C.pt_BR
dc.contributor.authorMantello C.C.pt_BR
dc.contributor.authorCampos T.pt_BR
dc.contributor.authorSouza L.M.pt_BR
dc.contributor.authorGoncalves P.S.pt_BR
dc.contributor.authorSouza A.P.pt_BR
unicamp.authorSilva, C.C., Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino VazCP 6010, Campinas, SP CEP 13083-970, Brazilpt_BR
unicamp.authorMantello, C.C., Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino VazCP 6010, Campinas, SP CEP 13083-970, Brazilpt_BR
unicamp.authorSouza, L.M., Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino VazCP 6010, Campinas, SP CEP 13083-970, Brazilpt_BR
unicamp.authorSouza, A.P., Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino VazCP 6010, Campinas, SP CEP 13083-970, Brazil, Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino VazCP 6109, Campinas, SP CEP 13083-970, Brazilpt_BR
unicamp.author.externalCampos, T., Centro de Pesquisa Agroflorestal do Acre (CPAFAC), Embrapa, Rodovia BR-364, km 14CP 321, Rio Branco, AC CEP 69900-970, Brazilpt
unicamp.author.externalGonçalves, P.S., Instituto Agronômico de Campinas (IAC), CP 28, Campinas, SP CEP 13012-970, Brazilpt
dc.description.abstractHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early evaluation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasiliensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. © 2014 The Author(s).en
dc.relation.ispartofMolecular Breedingpt_BR
dc.date.issued2014pt_BR
dc.identifier.citationMolecular Breeding. , v. , n. , p. - , 2014.pt_BR
dc.language.isoenpt_BR
dc.rightsfechadopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn13803743pt_BR
dc.identifier.doi10.1007/s11032-014-0095-2pt_BR
dc.identifier.urlhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84901727437&partnerID=40&md5=0cbbb32622dd55e95bbf26ec72e71875pt_BR
dc.date.available2015-06-25T17:51:34Z
dc.date.available2015-11-26T14:08:00Z-
dc.date.accessioned2015-06-25T17:51:34Z
dc.date.accessioned2015-11-26T14:08:00Z-
dc.description.provenanceMade available in DSpace on 2015-06-25T17:51:34Z (GMT). No. of bitstreams: 1 2-s2.0-84901727437.pdf: 822209 bytes, checksum: b1fe10ed57f8e51452cea70d652aa47c (MD5) Previous issue date: 2014en
dc.description.provenanceMade available in DSpace on 2015-11-26T14:08:00Z (GMT). No. of bitstreams: 2 2-s2.0-84901727437.pdf: 822209 bytes, checksum: b1fe10ed57f8e51452cea70d652aa47c (MD5) 2-s2.0-84901727437.pdf.txt: 74803 bytes, checksum: 1e585a1e9e8ae8b4ed5798c2d0fe5163 (MD5) Previous issue date: 2014en
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/86099
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/86099-
dc.identifier.idScopus2-s2.0-84901727437pt_BR
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