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dc.typeArtigo de periódicopt_BR
dc.titleHaemophilus Influenzae Porine Ompp2 Gene Transfer Mediated By Graphene Oxide Nanoparticles With Effects On Transformation Process And Virulence Bacterial Capacitypt_BR
dc.contributor.authorVarela J.N.pt_BR
dc.contributor.authorAmstalden M.C.K.pt_BR
dc.contributor.authorPereira R.F.C.pt_BR
dc.contributor.authorde Hollanda L.M.pt_BR
dc.contributor.authorCeragioli H.J.pt_BR
dc.contributor.authorBaranauskas V.pt_BR
dc.contributor.authorLancellotti M.pt_BR
unicamp.authorVarela, J.N., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
unicamp.authorAmstalden, M.C.K., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
unicamp.authorPereira, R.F.C., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
unicamp.authorde Hollanda, L.M., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
unicamp.authorCeragioli, H.J., NanoEng - NanoEngineering and Diamond Laboratory, School of Electrical and Computer Engineering, Department of Semiconductors, Instruments and Photonics, University of Campinas, UNICAMP, Av. Albert Einstein N.400, CEP 13 083-852 Campinas, São Paulo, Brazilpt_BR
unicamp.authorBaranauskas, V., NanoEng - NanoEngineering and Diamond Laboratory, School of Electrical and Computer Engineering, Department of Semiconductors, Instruments and Photonics, University of Campinas, UNICAMP, Av. Albert Einstein N.400, CEP 13 083-852 Campinas, São Paulo, Brazilpt_BR
unicamp.authorLancellotti, M., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
dc.description.abstractBackground: H. influenzae is a natural competent bacterium that can uptake DNA from the environment and recombine into bacterial genome. The outbreaks of Brazilian purpuric fever, heavily polluted areas of a different H. influenzae biogroup - aegyptius - as well as gene transference between Neisseria meningitis make the transformation process an important evolutionary factor. This work studied the horizontal transference of the ompP2 gene from a multiresistant strain of H. influenzae 07 (NTHi), under the influence of graphene oxide nanoparticles in order to mimic an atmosphere rich in suspended particles and this way verify if the CFU transformants number was increased.Material and methods: In this article the gene ompP2 was transformed into different strains of H. influenzae mediated or not by graphene oxide nanoparticles in suspension, followed by the adhesion tests in Hec-1B (human endometrium adenocarcinoma) and A549 (pulmonary epithelial carcinoma) cells lines. The transformation frequency and the adhesion capacity were determined in all the mutants to which the ompP2 gene was transferred and compared to their wild type strains.Results: The nanoparticles increased the transformation ratio of one particular strain isolated from a pneumonia case. The adhesion patterns to A549 and Hec1b cell lines of these mutated bacteria has their capacity increased when compared to the wild type.Conclusions: Graphene oxide nanoparticles aid the transformation process, helping to increase the number of CFUs, and the mutants generated with the ompP2 gene from a H. influenzae resistant strain not only present a chloramphenicol resistance but also have an increased adherence patterns in A549 and Hec1B cell lines. © 2014 Varela et al.; licensee BioMed Central Ltd.en
dc.relation.ispartofJournal of Nanobiotechnologypt_BR
dc.publisherBioMed Central Ltd.pt_BR
dc.identifier.citationJournal Of Nanobiotechnology. Biomed Central Ltd., v. 12, n. 1, p. - , 2014.pt_BR
dc.description.provenanceMade available in DSpace on 2015-06-25T17:52:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.description.provenanceMade available in DSpace on 2015-11-26T14:17:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.description.referenceWolf, J., Daley, A.J., Microbiological aspects of bacterial lower respiratory tract illness in children: typical pathogens (2007) Paediatr Respir Rev, 8, pp. 204-210. , quiz 210-201, 10.1016/j.prrv.2007.08.002, 17868918pt_BR
dc.description.referenceHolt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T., Facultatively Anaerobic Gram-negative Rods (1994) Bergey's Manual of Determinative Bacteriology, pp. 175-290. , Baltimore: Williams & Wilkins, 9pt_BR
dc.description.referenceKroll, J.S., Langford, P.R., Loynds, B.M., Copper-zinc superoxide dismutase of Haemophilus influenzae and H. parainfluenzae (1991) J Bacteriol, 173, pp. 7449-7457. , 212509, 1938942pt_BR
dc.description.referenceGilsdorf, J.R., Marrs, C.F., Foxman, B., Haemophilus influenzae: genetic variability and natural selection to identify virulence factors (2004) Infect Immun, 72, pp. 2457-2461. , 10.1128/IAI.72.5.2457-2461.2004, 387884, 15102751pt_BR
dc.description.referenceRahman, M., Hossain, S., Baqui, A.H., Shoma, S., Rashid, H., Nahar, N., Zaman, M.K., Khatun, F., Haemophilus influenzae type-b and non-b-type invasive diseases in urban children (<5 years) of Bangladesh: implications for therapy and vaccination (2008) J Infect, 56, pp. 191-196. , 10.1016/j.jinf.2007.12.008, 18280571pt_BR
dc.description.referenceBae, S., Lee, J., Kim, E., Lee, S., Yu, J., Kang, Y., Antimicrobial resistance in Haemophilus influenzae respiratory tract isolates in Korea: results of a nationwide acute respiratory infections surveillance (2010) Antimicrob Agents Chemother, 54, pp. 65-71. , 10.1128/AAC.00966-09, 2798543, 19884366pt_BR
dc.description.referenceTurk, D.C., The pathogenicity of Haemophilus influenzae (1984) J Med Microbiol, 18, pp. 1-16. , 10.1099/00222615-18-1-1, 6146721pt_BR
dc.description.referenceBooy, R., Heath, P.T., Slack, M.P., Begg, N., Moxon, E.R., Vaccine failures after primary immunisation with Haemophilus influenzae type-b conjugate vaccine without booster (1997) Lancet, 349, pp. 1197-1202. , 10.1016/S0140-6736(96)06392-1, 9130940pt_BR
dc.description.referenceWang, S.R., Tseng, M.H., Lin, W.J., Teng, C.S., Wang, C.C., Fatal non-typeable Haemophilus influenzae sepsis complicated with acute respiratory distress syndrome: case report and literature review (2005) Scand J Infect Dis, 37, pp. 921-925. , 10.1080/00365540500262492, 16308233pt_BR
dc.description.referenceBarenkamp, S.J., Munson, R.S., Granoff, D.M., Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles (1981) J Infect Dis, 143, pp. 668-676. , 10.1093/infdis/143.5.668, 6972422pt_BR
dc.description.referenceFoxwell, A.R., Kyd, J.M., Cripps, A.W., Nontypeable Haemophilus influenzae: pathogenesis and prevention (1998) Microbiol Mol Biol Rev, 62, pp. 294-308. , 98916, 9618443pt_BR
dc.description.referenceMurphy, T.F., Bartos, L.C., Purification and analysis with monoclonal antibodies of P2, the major outer membrane protein of nontypable Haemophilus influenzae (1988) Infect Immun, 56, pp. 1084-1089. , 259766, 2451640pt_BR
dc.description.referenceMurphy, T.F., Bartos, L.C., Human bactericidal antibody response to outer membrane protein P2 of nontypeable Haemophilus influenzae (1988) Infect Immun, 56, pp. 2673-2679. , 259628, 2843467pt_BR
dc.description.referenceArbing, M.A., Hanrahan, J.W., Coulton, J.W., Altered channel properties of porins from Haemophilus influenzae: isolates from cystic fibrosis patients (2002) J Membr Biol, 189, pp. 131-141. , 10.1007/s00232-002-1008-6, 12235488pt_BR
dc.description.referenceRegelink, A.G., Dahan, D., Moller, L.V., Coulton, J.W., Eijk, P., Van Ulsen, P., Dankert, J., Van Alphen, L., Variation in the composition and pore function of major outer membrane pore protein P2 of Haemophilus influenzae from cystic fibrosis patients (1999) Antimicrob Agents Chemother, 43, pp. 226-232. , 89055, 9925510pt_BR
dc.description.referenceGazagne, L., Delmas, C., Bingen, E., Dabernat, H., Molecular epidemiology of ampicillin-resistant non-beta-lactamase-producing Haemophilus influenzae (1998) J Clin Microbiol, 36, pp. 3629-3635. , 105253, 9817886pt_BR
dc.description.referenceYamanaka, N., Hotomi, M., Billal, D.S., Clinical bacteriology and immunology in acute otitis media in children (2008) J Infect Chemother, 14, pp. 180-187. , 10.1007/s10156-007-0599-3, 18574652pt_BR
dc.description.referenceDimopoulou, I.D., Kraak, W.A., Anderson, E.C., Nichols, W.W., Slack, M.P., Crook, D.W., Molecular epidemiology of unrelated clusters of multiresistant strains of Haemophilus influenzae (1992) J Infect Dis, 165, pp. 1069-1075. , 10.1093/infdis/165.6.1069, 1583325pt_BR
dc.description.referenceDimopoulou, I.D., Jordens, J.Z., Legakis, N.J., Crook, D.W., A molecular analysis of Greek and UK Haemophilus influenzae conjugative resistance plasmids (1997) J Antimicrob Chemother, 39, pp. 303-307. , 10.1093/jac/39.3.303, 9096178pt_BR
dc.description.referenceLeaves, N.I., Dimopoulou, I., Hayes, I., Kerridge, S., Falla, T., Secka, O., Adegbola, R.A., Crook, D.W., Epidemiological studies of large resistance plasmids in Haemophilus (2000) J Antimicrob Chemother, 45, pp. 599-604. , 10.1093/jac/45.5.599, 10797080pt_BR
dc.description.referenceCerquetti, M., Ciofi degli Atti, M.L., Cardines, R., Giufre, M., Romano, A., Mastrantonio, P., Haemophilus influenzae serotype e meningitis in an infant (2004) Clin Infect Dis, 38, p. 1041. , 10.1086/382083, 15034842pt_BR
dc.description.referenceBurns, J.L., Mendelman, P.M., Levy, J., Stull, T.L., Smith, A.L., A permeability barrier as a mechanism of chloramphenicol resistance in Haemophilus influenzae (1985) Antimicrob Agents Chemother, 27, pp. 46-54. , 10.1128/AAC.27.1.46, 176203, 3872625pt_BR
dc.description.referenceTakahata, S., Ida, T., Senju, N., Sanbongi, Y., Miyata, A., Maebashi, K., Hoshiko, S., Horizontal gene transfer of ftsI, encoding penicillin-binding protein 3, in Haemophilus influenzae (2007) Antimicrob Agents Chemother, 51, pp. 1589-1595. , 10.1128/AAC.01545-06, 1855551, 17325223pt_BR
dc.description.referenceMaughan, H., Redfield, R.J., Tracing the evolution of competence in Haemophilus influenzae (2009) PLoS One, 4, pp. e5854. , 10.1371/journal.pone.0005854, 2689351, 19516897pt_BR
dc.description.referenceChen, I., Dubnau, D., DNA uptake during bacterial transformation (2004) Nat Rev Microbiol, 2, pp. 241-249. , 10.1038/nrmicro844, 15083159pt_BR
dc.description.referenceMaughan, H., Redfield, R.J., Extensive variation in natural competence in Haemophilus influenzae (2009) Evolution, 63, pp. 1852-1866. , 10.1111/j.1558-5646.2009.00658.x, 19239488pt_BR
dc.description.referenceZhao QQB, A., CHOWDHRY, U., Nanotechnology in the Chemical Industry - Opportunities and Challenges (2003) J nanoparticles Res, 5, pp. 567-572pt_BR
dc.description.referenceRojas-Chapana, J., Troszczynska, J., Firkowska, I., Morsczeck, C., Giersig, M., Multi-walled carbon nanotubes for plasmid delivery into Escherichia coli cells (2005) Lab Chip, 5, pp. 536-539. , 10.1039/b500681c, 15856091pt_BR
dc.description.referenceMattos, I.B., Alves, D.A., Hollanda, L.M., Ceragiogli, H.J., Baranauskas, V., Lancellotti, M., Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process (2011) J Nanobiotechnol, 9, p. 53pt_BR
dc.description.referenceHollanda, L.M., Cury, G.C., Pereira, R.F., Ferreira, G.A., Sousa, A., Sousa, E.M., Lancellotti, M., Effect of mesoporous silica under Neisseria meningitidis transformation process: environmental effects under meningococci transformation (2011) J Nanobiotechnol, 9, p. 28pt_BR
dc.description.referenceBorenfreund, E., Borrero, O., In vitro cytotoxicity assays. Potential alternatives to the Draize ocular allergy test (1984) Cell Biol Toxicol, 1, pp. 55-65. , 10.1007/BF00125565, 6400923pt_BR
dc.description.referencePereira RFC, A.D., Jacinto, R.K., Hollanda, L.M., Verinaud, L.M.C., Machado, C.M.L., Lancellotti, M., Effects of Neisseria meningitidis Infection in Tumor Glioblastoma Cell Line NG97: Respiratory Pathogen Inducing Apoptosis (2011) J Bacteriol Parasitol, 2, p. 4pt_BR
dc.description.referenceSambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular cloning: a laboratory manual, , CSH Laboratory Presspt_BR
dc.description.referenceKonowalchuk, J., Speirs, J.I., Stravic, S., Vero response to a cytotoxin of Escherichia coli (1977) Infect Immun, 18, pp. 775-779. , 421302, 338490pt_BR
dc.description.referenceBabich, H., Puerner, J.A., Borenfreund, E., In vitro cytotoxicity of metals to bluegill (BF-2) cells (1986) Arch Environ Contam Toxicol, 15, pp. 31-37. , 10.1007/BF01055246, 3947137pt_BR
dc.description.referenceScaletsky, I.C., Silva, M.L., Trabulsi, L.R., Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells (1984) Infect Immun, 45, pp. 534-536. , 263286, 6146569pt_BR
dc.description.referenceKroll, J.S., Wilks, K.E., Farrant, J.L., Langford, P.R., Natural genetic exchange between Haemophilus and Neisseria: intergeneric transfer of chromosomal genes between major human pathogens (1998) Proc Natl Acad Sci U S A, 95, pp. 12381-12385. , 10.1073/pnas.95.21.12381, 22840, 9770495pt_BR
dc.description.referenceRibeiro, H., Assunção, J.V., Efeitos das queimadas na saúde humana (2002) Estud Avançados, 16, p. 24pt_BR
dc.description.referenceFoster, K.A., Oster, C.G., Mayer, M.M., Avery, M.L., Audus, K.L., Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism (1998) Exp Cell Res, 243, pp. 359-366. , 10.1006/excr.1998.4172, 9743595pt_BR
dc.description.referenceSpence, J.M., Clark, V.L., Role of ribosomal protein L12 in gonococcal invasion of Hec1B cells (2000) Infect Immun, 68, pp. 5002-5010. , 10.1128/IAI.68.9.5002-5010.2000, 101722, 10948117pt_BR
dc.description.referenceBenson, S.A., Occi, J.L., Sampson, B.A., Mutations that alter the pore function of the OmpF porin of Escherichia coli K12 (1988) J Mol Biol, 203, pp. 961-970. , 10.1016/0022-2836(88)90121-0, 2463371pt_BR
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