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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleHaemophilus Influenzae Porine Ompp2 Gene Transfer Mediated By Graphene Oxide Nanoparticles With Effects On Transformation Process And Virulence Bacterial Capacitypt_BR
dc.contributor.authorVarela J.N.pt_BR
dc.contributor.authorAmstalden M.C.K.pt_BR
dc.contributor.authorPereira R.F.C.pt_BR
dc.contributor.authorde Hollanda L.M.pt_BR
dc.contributor.authorCeragioli H.J.pt_BR
dc.contributor.authorBaranauskas V.pt_BR
dc.contributor.authorLancellotti M.pt_BR
unicamp.authorVarela, J.N., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
unicamp.authorAmstalden, M.C.K., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
unicamp.authorPereira, R.F.C., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
unicamp.authorde Hollanda, L.M., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
unicamp.authorCeragioli, H.J., NanoEng - NanoEngineering and Diamond Laboratory, School of Electrical and Computer Engineering, Department of Semiconductors, Instruments and Photonics, University of Campinas, UNICAMP, Av. Albert Einstein N.400, CEP 13 083-852 Campinas, São Paulo, Brazilpt_BR
unicamp.authorBaranauskas, V., NanoEng - NanoEngineering and Diamond Laboratory, School of Electrical and Computer Engineering, Department of Semiconductors, Instruments and Photonics, University of Campinas, UNICAMP, Av. Albert Einstein N.400, CEP 13 083-852 Campinas, São Paulo, Brazilpt_BR
unicamp.authorLancellotti, M., LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazilpt_BR
dc.description.abstractBackground: H. influenzae is a natural competent bacterium that can uptake DNA from the environment and recombine into bacterial genome. The outbreaks of Brazilian purpuric fever, heavily polluted areas of a different H. influenzae biogroup - aegyptius - as well as gene transference between Neisseria meningitis make the transformation process an important evolutionary factor. This work studied the horizontal transference of the ompP2 gene from a multiresistant strain of H. influenzae 07 (NTHi), under the influence of graphene oxide nanoparticles in order to mimic an atmosphere rich in suspended particles and this way verify if the CFU transformants number was increased.Material and methods: In this article the gene ompP2 was transformed into different strains of H. influenzae mediated or not by graphene oxide nanoparticles in suspension, followed by the adhesion tests in Hec-1B (human endometrium adenocarcinoma) and A549 (pulmonary epithelial carcinoma) cells lines. The transformation frequency and the adhesion capacity were determined in all the mutants to which the ompP2 gene was transferred and compared to their wild type strains.Results: The nanoparticles increased the transformation ratio of one particular strain isolated from a pneumonia case. The adhesion patterns to A549 and Hec1b cell lines of these mutated bacteria has their capacity increased when compared to the wild type.Conclusions: Graphene oxide nanoparticles aid the transformation process, helping to increase the number of CFUs, and the mutants generated with the ompP2 gene from a H. influenzae resistant strain not only present a chloramphenicol resistance but also have an increased adherence patterns in A549 and Hec1B cell lines. © 2014 Varela et al.; licensee BioMed Central Ltd.en
dc.relation.ispartofJournal of Nanobiotechnologypt_BR
dc.publisherBioMed Central Ltd.pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationJournal Of Nanobiotechnology. Biomed Central Ltd., v. 12, n. 1, p. - , 2014.pt_BR
dc.language.isoenpt_BR
dc.description.volume12pt_BR
dc.description.issuenumber1pt_BR
dc.rightsabertopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn14773155pt_BR
dc.identifier.doi10.1186/1477-3155-12-14pt_BR
dc.identifier.urlhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84899913557&partnerID=40&md5=3451b0529c692386311ee29d6ab142b4pt_BR
dc.date.available2015-06-25T17:52:35Z
dc.date.available2015-11-26T14:17:45Z-
dc.date.accessioned2015-06-25T17:52:35Z
dc.date.accessioned2015-11-26T14:17:45Z-
dc.description.provenanceMade available in DSpace on 2015-06-25T17:52:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.description.provenanceMade available in DSpace on 2015-11-26T14:17:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/86298
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/86298-
dc.identifier.idScopus2-s2.0-84899913557pt_BR
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