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Type: Artigo de periódico
Title: Karyotype Characterization Of Two Populations Of Vernonia Geminata (asteraceae, Vernonieae) Using Banding And Fish Techniques.
Author: Oliveira V.M.
Mansanares M.E.
Semir J.
Forni-Martins E.R.
Abstract: In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA(3)(+) terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA(+) and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes.
Citation: Genetics And Molecular Research : Gmr. , v. 11, n. 4, p. 4204 - 4212, 2012.
Rights: fechado
Identifier DOI: 10.4238/2012.September.25.1
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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