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Type: Artigo de periódico
Title: Chemical And Photochemical Generated Carbon-centered Radical Intermediate And Its Reaction With Desoxyribonucleic Acid
Author: Justo G.Z.
Livotto P.R.
Duran N.
Abstract: The possible action of carbon-centered radicals in promoting damage to DNA is explored by generation of the 2-phenylethyl radical. The radical is generated during oxidation of phenelzine (2-phenylethyl hydrazine) by ferricyanide, as well as optically from phenylpropionic acid. Covalent binding of the 2-phenylethyl radical to DNA is suggested by studies with the plasmid pBR 322 DNA. Other sensitive techniques used to study DNA damage were the interaction with formaldehyde at 60°C and the fluorescence of DNA-Th(III) and DNA-DAPI complexes. Theoretical MNDO calculations indicated a preferential attack at position 8 of the guanine residues. This study shows that the 2-phenylethyl radical is able to induce primary effects on nucleic acid structure, leading to alkylated products, especially at purine rings. © 1995.
Citation: Free Radical Biology And Medicine. , v. 19, n. 4, p. 431 - 440, 1995.
Rights: fechado
Identifier DOI: 10.1016/0891-5849(95)00036-W
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

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