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dc.typeArtigo de periódicopt_BR
dc.titleExopolysaccharide Matrix Of Developed Candida Albicans Biofilms After Exposure To Antifungal Agentspt_BR
dc.contributor.authorDa Silva W.J.pt_BR
dc.contributor.authorGoncalves L.M.pt_BR
dc.contributor.authorSeneviratne J.pt_BR
dc.contributor.authorParahitiyawa N.pt_BR
dc.contributor.authorSamaranayake L.P.pt_BR
dc.contributor.authorDel Bel Cury A.A.pt_BR
unicamp.authorDa Silva, W.J., Department of Prosthodontics and Periodontology, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazilpt_BR
unicamp.authorGonçalves, L.M., Department of Prosthodontics and Periodontology, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazilpt_BR
unicamp.authorDel Bel Cury, A.A., Department of Prosthodontics and Periodontology, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazilpt_BR, J., Oral Biosciences, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kongpt, N., Faculty of Medicine, University of Peradeniya, Colombo, Sri Lankapt, L.P., Oral Biosciences, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kongpt
dc.description.abstractThis study aimed to evaluate the effects of fluconazole or nystatin exposure on developed Candida albicans biofilms regarding their exopolysaccharide matrix. The minimal inhibitory concentration (MIC) against fluconazole or nystatin was determined for C. albicans reference strain (ATCC 90028). Poly(methlymethacrylate) resin (PMMA) specimens were fabricated according to the manufacturer's instructions and had their surface roughness measured. Biofilms were developed on specimens surfaces for 48 h and after that were exposed during 24 h to fluconazole or nystatin prepared in a medium at MIC, 10 x MIC or 100 x MIC. Metabolic activity was evaluated using an XTT assay. Production of soluble and insoluble exopolysaccharide and intracellular polysaccharides was evaluated by the phenol-sulfuric method. Confocal laser scanning microscope was used to evaluate biofilm architecture and percentage of dead/live cells. Data were analyzed statistically by ANOVA and Tukey's test at 5% significance level. The presence of fluconazole or nystatin at concentrations higher than MIC results in a great reduction of metabolic activity (p<0.001). At MIC or 10 x MIC, fluconazole showed high amounts of intracellular polysaccharides (p<0.05), but did not affect the exopolysaccharide matrix (p>0.05). The exposure to nystatin also did not alter the exopolysaccharide matrix at all the tested concentrations (p>0.05). Biofilm architecture was not affected by either of the antifungal agents (p>0.05). Nystatin promoted higher proportion of dead cells (p<0.05). It may be concluded that fluconazole and nystatin above the MIC concentration reduced the metabolic activity of C. albicans biofilms; however, they were not able to alter the exopolysaccharide matrix and biofilm architecture.en
dc.relation.ispartofBrazilian Dental Journalpt_BR
dc.identifier.citationBrazilian Dental Journal. , v. 23, n. 6, p. 716 - 722, 2012.pt_BR
dc.description.provenanceMade available in DSpace on 2015-06-26T20:29:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.description.provenanceMade available in DSpace on 2015-11-26T14:25:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
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