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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleInvestigation Of The Cytocidal Potential Of Rhinella Jimi Skin Methanol Extractspt_BR
dc.contributor.authorBrito S.V.pt_BR
dc.contributor.authorSales D.L.pt_BR
dc.contributor.authorCosta J.G.M.pt_BR
dc.contributor.authorRodrigues F.F.G.pt_BR
dc.contributor.authorFerreira F.S.pt_BR
dc.contributor.authorAngelico E.C.pt_BR
dc.contributor.authorCarvalho J.E.pt_BR
dc.contributor.authorAlmeida W.O.pt_BR
dc.contributor.authorAnjos L.A.pt_BR
dc.contributor.authorCoutinho H.D.M.pt_BR
unicamp.authorCarvalho, J.E., Universidade Estadual de Campinas - UNICAMP, Campinas, SP, Brazilpt_BR
unicamp.author.externalBrito, S.V., Universidade Federal da Paraíba - UFPB, João Pessoa, PB, Brazilpt
unicamp.author.externalSales, D.L., Departamento de Química Biológica, Universidade, Regional do Cariri - URCA, Av. Cel. Antônio Luiz, 1161, CEP:63105-000, Crato, CE, Brazilpt
unicamp.author.externalCosta, J.G.M., Departamento de Química Biológica, Universidade, Regional do Cariri - URCA, Av. Cel. Antônio Luiz, 1161, CEP:63105-000, Crato, CE, Brazilpt
unicamp.author.externalRodrigues, F.F.G., Departamento de Química Biológica, Universidade, Regional do Cariri - URCA, Av. Cel. Antônio Luiz, 1161, CEP:63105-000, Crato, CE, Brazilpt
unicamp.author.externalFerreira, F.S., Universidade Federal da Paraíba - UFPB, João Pessoa, PB, Brazilpt
unicamp.author.externalAngélico, E.C., Departamento de Química Biológica, Universidade, Regional do Cariri - URCA, Av. Cel. Antônio Luiz, 1161, CEP:63105-000, Crato, CE, Brazilpt
unicamp.author.externalAlmeida, W.O., Universidade Federal da Paraíba - UFPB, João Pessoa, PB, Brazilpt
unicamp.author.externalAnjos, L.A., Universidade Estadual Paulista - UNESP, Botucatu, SP, Brazilpt
unicamp.author.externalCoutinho, H.D.M., Departamento de Química Biológica, Universidade, Regional do Cariri - URCA, Av. Cel. Antônio Luiz, 1161, CEP:63105-000, Crato, CE, Brazilpt
dc.description.abstractContext: Amphibian skins have wide variety of biologically active compounds associated with the natural defenses of these animals. Objectives: To study the in vitro anticancer activity of methanol extracts of the skin of Rhinella jimi Stevaux (Anura: Bufonidae). Material and methods: The extract was obtained by cold methanol extraction for 96 h using dried skins (295 mg). The methanol skin extract was dried under reduced pressure, giving a 5.5% yield. In order to test for growth-inhibitory activity, in vitro tests were performed with the following cancer cell lines using concentrations ranging between 0.25-250 μg/mL of the extract by 48 h: K562 (leukemia), MCF-7 (breast), NCI-ADR (breast with MDR phenotype), UACC-62 (melanoma), NCI460 (lung), PCO3 (prostate), HT-29 (colon), OVCAR (ovary), and 786-0 (kidney). Results: The methanol extract of R. jimi produced a growth inhibition in a dose-dependent manner against the most of the assayed cell lines. In addition to the growth inhibition, the extract induced the cell death in the ovary and colon lines (EC50 0.125 and 0.2 μg/mL, respectively), demonstrating 100% of inhibition with 2.5 μg/mL. However, prostate and leukemia cell lines demonstrated less sensitivity, with EC50 of 24 and 235 μg/mL, respectively. This is the first report about the anticancer activity by natural products from the skin of R. jimi. Conclusions: The methanol extracts of R. jimi significantly affected the growth of several cell lines, demonstrating that these compounds are a potential source of substances that could be utilized in cancer treatments. © 2012 Informa Healthcare USA, Inc.en
dc.relation.ispartofPharmaceutical Biologypt_BR
dc.date.issued2012pt_BR
dc.identifier.citationPharmaceutical Biology. , v. 50, n. 8, p. 1026 - 1030, 2012.pt_BR
dc.language.isoenpt_BR
dc.description.volume50pt_BR
dc.description.issuenumber8pt_BR
dc.description.firstpage1026pt_BR
dc.description.lastpage1030pt_BR
dc.rightsfechadopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn13880209pt_BR
dc.identifier.doi10.3109/13880209.2012.655858pt_BR
dc.identifier.urlhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84863702417&partnerID=40&md5=c29cb27b5a5eabbe063913e6164e18f6pt_BR
dc.date.available2015-06-26T20:30:37Z
dc.date.available2015-11-26T14:32:04Z-
dc.date.accessioned2015-06-26T20:30:37Z
dc.date.accessioned2015-11-26T14:32:04Z-
dc.description.provenanceMade available in DSpace on 2015-06-26T20:30:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.description.provenanceMade available in DSpace on 2015-11-26T14:32:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/97401
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/97401-
dc.identifier.idScopus2-s2.0-84863702417pt_BR
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